中文摘要：

本试验旨在建立利用高效液相色谱同时测定瘤胃液中几种水溶性维生素的方法。取瘤胃液，用正己烷、偏磷酸萃取后，其上清液用氮气吹干浓缩（40℃），以流动相A溶解。色谱条件：SBC8色谱柱，粒径5μm，4．6mm×150mm；流动相A，V甲醇：V0.05mol／L磷酸三钠（pH=2.5）=7：93；流动相B，V甲醇：V0.05mol／L磷酸三钠（pH=2.5）=90：10；流速1．0mL／min；检测波长245nm。结果表明：1）该方法对烟酰胺、烟酸、叶酸、核黄素和氰钴胺素的最小检出质量浓度分别为12．90、12．00、74．11、24．00、9．57ng／mL（信噪比=3），线性回归相关系数分别为0．9999、0．9993、0．9903、0．9990、0．9995；2）瘤胃液样品中加标烟酰胺、烟酸、叶酸、核黄素和氰钴胺素的平均回收率分别为97．5％、96．8％、73．2％、84．4％、89．1％。相对标准偏差分别为2．9％、3．0％、15．6％、7．8％、5．0％。由此得出，本方法适用于同时测定瘤胃液中烟酰胺和烟酸的含量。

英文摘要：

This experiment was conducted to establish a method for simultaneous determination of several wa- ter-soluble vitamins in rumen fluid by high performance liquid chromatographic （HPLC）. Rumen fluid sample was extracted with hexane and metaphosphoric acid, and the supernatant was evaporated in a nitrogen stream at 40 ℃. The concentrate was dissolved by mobile phase A. Chromatographic condition was the following： SBC8 column, particle side 5 μm, 4.6 mm × 150 mm; mobile phase A, Vmethanol ： V0.05 mol/L trisodium phosphate （pH =2.5） =7 ： 93 ; mobile phase B, Vmethanol： V0.05 moVE trisodium phosphate （pn =2.5） = 90 ： 10 ; flOW rate was 1.0 mL/ min ; detection wave- length was 245 nm. The results showed as follows ： 1 ） the minimum detecting concentrations of niacinamide, nicotinic acid, folic, riboflavin acid and cyanocobalamin were 12. 90, 12. 00, 74. 11, 24. 00 and 9.57 ng/mL （ signal to noise ratio =3 ）, and correlation coefficients of linear regression were 0. 999 9, 0. 999 3, 0. 990 3, 0. 999 0 and 0. 999 5, respectively ; 2 ） the average recoveries of adding standard niaci- namide, nicotinic acid, folic acid, riboflavin and cyanocobalamin in rumen fluid were 97. 5%, 96.8%, 73.2%, 84.4% and 89.1%, and the average relative standard deviations of those were 2.9%, 3.0%, 15.6%, 7.8% and 5.0%, respectively. The results indicate that the contents of niacinamide and nicotinic acid in rumen fluid can be simultaneously determined by this method. [ Chinese Journal of Animal Nutrition,