中文摘要：

目的：探讨侯氏黑散方中风药、补虚药以及风药补虚药联用对脑缺血大鼠轴突生长抑制因子Nogo-A、NgR、RhoA、Rock2蛋白表达的影响。方法采取线栓法制备永久性大脑中动脉栓塞（pMACO）模型。大鼠随机分为假手术组、模型组、风药组（7．70 g／kg）、补虚药组（2．59 g／kg）、风药补虚药联用组（10．50 g／kg）。术后每12 h 灌胃给药1次，连续给药72 h。运用 Western blot 检测大鼠缺血侧海马 CA1区 Nogo-A、NgR、RhoA、Rock2蛋白表达。结果与假手术组相比，模型组大鼠 Nogo-A、NgR、RhoA、Rock2蛋白的表达均升高（P ＜0．01）；与模型组相比，各给药组大鼠 NgR 的蛋白表达均显著下降（P ＜0．01）；RhoA、Rock2蛋白的表达均不同程度的下降，但只有联用药组具有统计学差异（P ＜0．05）。结论侯氏黑散中风药、补虚药可能通过对脑缺血大鼠轴突生长抑制信号通路 Nogo-A ／RhoA ／Rock2的调节促进脑缺血后神经功能的修复。

英文摘要：

Objective To explore the effect of wind-dispelling drugs and deficiency-tonifying drugs of Hou’s Hei San,a TCMformula used in treatment of apoplexy,on axonal growth inhibitory factors Nogo-A,NgR,RhoA,Rock2 of rats with cerebral ischemia.Methods The model of permanent middle cere-bral artery occlusion （pMCAO）was induced by using Longa method.All rats were randomly divided in-to sham-operation group,model group,wind-dispelling drugs group（7.70 g/kg）,deficiency-tonifying drugs group（2.59 g/kg）and combined drugs group（10.50 g/kg）.After operation,each 12 h the rats were intragastrically administered for 72 h.Then the rats were sacrificed and their brain tissue were re-sected.The protein expression of Nogo-A,NgR,RhoA and Rock2 in hippocampus CA1 were detected by using Western blot.Results The protein expressions of Nogo-A,NgR,RhoA and Rock2 in model group increased significantly,compared with sham-operation group（P 〈0.01 ）.Compared with model group, the expression of NgR in wind-dispelling drugs group,deficiency-tonifying drugs group and combined drugs group decreased remarkably（P 〈0.01）.The expression of Nogo-A and Rock2 decreased as well, but only combined drugs group showed a statistically difference（P 〈0.05）.Conclusion The effect of＆amp;nbsp;wind-dispelling drugs and deficiency-tonifying drugs of Hou’s Hei San against cerebral ischemia damage would be via regulating axonal growth inhibitory signal pathway Nogo-A /RhoA /Rock2 to promote the re-covery of neural function.