中文摘要：

目的探讨尿苷二磷酸葡萄糖醛酸转移酶1A91399C〉T（UGT1A91399C〉T）单核苷酸多态性对乳腺手术患者异丙酚镇静效应的影响。方法择期全麻下行良性乳腺肿块切除术的女性患者152例，年龄20—50岁，体重50—70kg，ASA分级I级或Ⅱ级。术前采用基因测序技术进行UGTlA91399单核苷酸多态性位点的检测，根据基因型将患者分为野生纯合子（C／C）组、突变杂合子（C／T）组和突变纯合子（T／T）组。麻醉诱导和维持时均靶控输注异丙酚，cp3,ug／ml，输注60min时采集血样，采用高效液相色谱法测定血浆异丙酚浓度。记录停止输注异丙酚至警觉．镇静（OAAS）评分达4分的时间，此时的BIS值和异丙酚效应室浓度；BIS值升至80的时间及此时异丙酚效应室浓度。结果野生纯合组24例，突变杂合组96例和突变纯合组32例。等位基因频率T为53％，c为47％。三组患者血浆异丙酚浓度、OAAS评分降至4分时的时间、BIS值、异丙酚效应室浓度和BIS值升至80时的时间、异丙酚效应室浓度比较差异无统计学意义（P〉0．05）。结论UGTlA91399C〉T单核苷酸多态性不是引起异丙酚镇静效应个体差异的遗传因素。

英文摘要：

Objective To investigate the effects of UDP glucuronosyhransferase （UGT） 1A9 I399 C 〉 T single nucleotide polymorphism on postoperative sedation with propofol in patients undergoing breast surgery. Methods One hundred and fifty-two ASA I or Ⅱ female patients, aged 20-50 yr, weighing 50-70 kg, scheduled for elective benign breast tumor excision under general anesthesia, were enrolled in this study. The polymorphic sites of the UGT1A9 I399 C 〉 T allele were analyzed by polymerase chain reactionestriction fragment length polymorphism. The patients were assigned to one of 3 groups according to their genotypes： wild homozygote （C/C） group, mutation heterozygote （C/T） group and mutation homozygote （T/T） group. During induction and maintenance of anesthesia, propofol was given by target-controlled infusion with the plasma concentration （Cp） of 3 tLg/ml. Blood samples were taken at 60 min after target-controlled infusion of propofol was started for determination of the Cp of propofol using high-performance liquid chromatography. The time when OAAS was 4 after stopping the infusion of propofol was recorded and the BIS value and effect-site concentration of propofol were also recorded at this time. The time when BIS value was 80 was recorded and the effect-site concentration of propofol was also recorded at this time. Results Genotyping analysis revealed that genotype distribution of UGT1 A9 1399 C 〉 T polymorphism wasC/C 24 cases, C/T 96 cases and T/T 32 cases. The T allele frequency was 53 %. The C allele frequency was 47.4%. There was no significant difference in the Cp of propofol, time when OAAS was 4, BIS value and effectsite concentration of propofol when OAAS was d, time when BIS value was 80 and effect-site concentration of propotbl when BIS value was 80 among the three groups （ P 〉 0.05）. Conclusion UGT1 A9 I399C 〉 T single nucleotide polymorphism is not the＇ genetic factor contributing to the individual variation in the patient s response to postoperative analgesia with pr