中文摘要：

目的：研究明胶微粒（GP）粒径及明胶与磷酸钙骨水泥（CPC）质量配比这两大因素对GP／CPC复合人工骨材料修复骨缺损的效果。方法：将不同GP粒径（100-200μm、200-300μm）及不同GP与CPC配比（5％GP、10％GP）两两配对后制备GP／CPC复合材料，比较GP／CPC复合材料的孔隙率、抗压强度、表面超微结构、体外生物相容性四大理化特性；构建新西兰兔颅骨缺损模型，在相同质控条件下将上述4组GP／CPC复合人工骨材料进行骨缺损填充，观察并比较其骨修复组织学差异及新骨形成率差异。结果：与粒径100-200μm的GP和5％GP配比组比较，粒径200-300μm的GP和10％GP配比制备的GP／CPC复合材料具有较大的孔径和较高的孔隙率（ P＜0．05），且在兔颅骨缺损修复中有更高的新骨形成量（ P＜0．05）；而100-200μm粒径、5％GP配比组则具有较强的抗压强度（ P＜0．05）。结论：不同GP粒径和含量的GP／CPC复合材料理化特性和修复骨缺损的效果不同，其中GP 200-300μm粒径和10％质量比的GP／CPC复合新型材料更具成骨效果。

英文摘要：

Objective：To investigate the influence of gelatin particle （ GP） size and gelatin/calcium phosphate cement （ GP/CPC） ratio on repairing potency of comparison artificial bone material .Methods：Composite GP/CPC materials with different GP size （100 -200 μm vs.200 -300 μm ） and ratio （ 5% vs.10%） were prepared . Physiochemical and biological properties , including porosity , resistance to compression , ultrastructure and biocompatibility were compared among 4 groups of GP/CPC materials.Different GP/CPC materials were used to repair the critical-size cranial bone defect in rabbit model , and the histology and newly formed bone inside scaffolds （ nBIS） were examined and compared among different groups .Results： GP/CPC with GP of 200 -300 μm possessed larger micropores than that with GP of 100 -200μm （ P〈0 .05 ） .The GP/CPC containing 10%GP had higher porosity than that containing 5%GP（P〈0.05）.The animal model study showed that more new bone formed in those defects filled with GP/CPC containing 10% GP with 200 -300 μm in size compared with GP/CPC containing 5% GP with 100 -200 μm in size （ P 〈0.05 ） . While GP/CPC containing 5%GP with 100-200 μm in size showed a higher level of resistance to compression .Conclusion：Both the particle size of GP and its ratio in the GP/CPC affect the properties of the composite biomaterials and their role in bone repairing .In particular , the GP/CPC containing 10%GP with 200-300 μm in size is most suitable for repairing critical-size cranial bone defect in animal model .