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人朊蛋白C端铜结合位点三维精细结构以及与兔朊蛋白的对比
  • ISSN号：0253-2778
  • 期刊名称：《中国科学技术大学学报》
  • 时间：0
  • 分类：O434.19[机械工程—光学工程;理学—光学;理学—物理]
  • 作者机构：[1]中国科学技术大学国家同步辐射实验室,安徽合肥230029, [2]中国科学院上海药物研究所,上海201203, [3]中国科学院上海应用物理研究所上海同步辐射光源,上海201204, [4]厦门大学化学与化工学院福建省生化重点实验室,福建厦门361005
  • 相关基金：Supported by the Knowledge Innovation Program of the Chinese Academy of Sciences （KJCX2-YW-N42）, the Key Important Project of the National Natural Science Foundation of China （10734070）, the National Key Basic Research （973） Program of China （2009CB930804）, the National Natural Science Foundation of China （11179023, 11275227, 31170717）, the Fundamental Research Funds for the Central Universities （2310000044）.
作者： 崔培昕[1], 练富林[2], 王宇[3], 文祎[2], 储旺盛[1], 林东海[4], 吴自玉[1]
关键词： 人朊蛋白, 铜, X射线吸收谱, MXAN, 兔朊蛋白, HuPrP, copper, XANES, MXAN, RaPrP
中文摘要：

朊蛋白是铜结合蛋白，而铜离子介导了朊蛋白的二级结构变化，使其聚沉在中枢神经系统中，导致传染性海绵状脑病．重组人朊蛋白91—231截去了八肽重复区域，利用X射线吸收谱近边结构解析出了C端高亲和性的铜结合位点的三维精细结构．结果显示与兔朊蛋白相比人朊蛋白中氨基酸与铜结合更紧密．本研究可能揭示了不同物种对传染性海绵状脑病具有不同抗性的原因．

英文摘要：

Prion protein is considered to be a copper-related protein, and the copper ion induces the secondary structure changes of prion proteins, making them aggregate and deposit in the central nervous system, and causing transmissible spongiform eneephalopathies （TSE）. The three-dimensional fine structure of C-terminal high-affinity eopper binding site was determined in recombinant human prion 91-231 （HuPrP91-231）, which lacks the octapeptide repeat region, using X-ray absorption near-edge structure （XANES） combined with the ab initio calculations in the framework of the multiple-scattering theory. Results show that amino acid residues have closer distances from the copper ion compared with those of the rabbit prion. This might be the reason why different species have different immunities to TSE.

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期刊信息
  • 《中国科学技术大学学报》
  • 北大核心期刊（2011版）
  • 主管单位:中国科学院
  • 主办单位:中国科学技术大学
  • 主编：何多慧
  • 地址：安徽省合肥市金寨路96号
  • 邮编：230026
  • 邮箱：JUST@USTC.EDU.CN
  • 电话：0551-63601961 63607694
  • 国际标准刊号：ISSN：0253-2778
  • 国内统一刊号：ISSN：34-1054/N
  • 邮发代号:26-31
  • 获奖情况:
  • 1999年，全国优秀高等学校自然科学学报及教育部优...,2001年，安徽省1999-2001年度优秀科技期刊一等奖,2002年，第三届华东地区优秀期刊奖
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  • 被引量:8237