中文摘要：

目的研究慢性吗啡依赖和戒断大鼠远位触液神经元环腺苷酸反应元件结合蛋白（pCREB）表达的变化。方法雄性SD大鼠24只，随机分为3组（n=8）：空白对照组、慢性吗啡依赖组、慢性吗啡戒断组。采用侧脑室注射CB-HRP和CB—HRP／pCREB免疫组织化学双重标记技术，观察大鼠远位触液神经元pCREB表达的变化。结果慢性吗啡依赖、戒断后，远位触液神经元出现CREB蛋白激活，两组CB—HRP／pCREB双标的神经元数量分别为223．0±61．5和213．0±50．6；与空白对照组相比较，磷酸化的CREB出现表达上调（P〈0．01）。吗啡依赖组和戒断组的CB-HRP双标神经元的数量相比，差异无显著性（P〉O．05）。结论远位触液神经元通过上调磷酸化CREB的表达参与吗啡依赖和戒断的形成。

英文摘要：

Objective To investigate the changes in pCREB protein expression in the distal cerebrospinal fluid contacting neurons induced by chronic morphine dependence and withdrawal. Methods Twenty-four SD rats of both sexes were randomly divided into 3 groups （ n = 8） ：the control group;the chronic morphine dependence group;the chronic morphine abstinence group. The methods of intraventricular injection of CB-HRP and CB-HRP/pCREB double-labeling in immunohistochemistry methods were used to observe the expression of pCREB in the distal ccrebrospinal fluid contacting neurons. Results The CREB protein was activated in the distal cerebrospinal fluid contacting neurons induced by chronic morphine dependence and withdrawal, the numbers of CB-HRP double-labeling neurons in the two experimental groups were significantly elevated 223.0 ± 61.5 and 213.0 ± 50.6 respectively; when compared to the control expression of pCREB protein （ P 〈 0.01 ）. Chronic morphine dependence and withdrawal showed no differences on the expression of pCREB protein （ P 〉 0.05 ）. Conclusion The distal cerebrospinal fluid contacting neurons were involved in the formation of chronic morphine dependence and withdrawal by activating the CRBE protein.