以引种植物紫串扦插苗为试验材料,研究其在高温胁迫环境下形态结构和生理指标的变化,其目的是探明紫串对高温胁迫的生理响应及适应机制。结果显示,高温胁迫对紫串的形态特征产生了一定的影响,随着胁迫温度升高或胁迫时间延长,紫串受害指数均逐渐增大,持续高温胁迫可导致紫串植株萎蔫甚至死亡,解除胁迫后多数植株能够进行自我恢复;高温胁迫过程中紫串的大多数生理指标均呈一定的规律性变化,随着胁迫温度升高或胁迫时间延长,叶绿素和类胡萝卜素含量均明显减少,丙二醛和脯氨酸含量基本呈现微增-剧增-微减的趋势,SOD活性持续缓慢升高,CAT活性先缓慢上升而后急剧下降;35℃和40℃高温胁迫对紫串扦插苗生长影响较小,受害指数和大部分生理生化指标均与对照差异不显著,45℃高温胁迫1~2d后紫串会产生一定的热伤害,但可以通过自我调节进行恢复,45℃高温胁迫3d后只有45%的植株可通过自身调节继续存活,55%植株萎蔫甚至死亡;高温环境下紫串扦插苗受害指数与叶绿素、丙二醛﹑脯氨酸、SOD和CAT等生理指标间均具有显著相关性,可以作为紫串的耐热性鉴定指标。
The morphological and physiological changes was researched with Scaevola albida 'Mauve Clusters' cutting seedlings as materials in order to explore the physiological response and adapting mechanism of Mauve Clusters under high temperature stress in this study.Results showed that high temperature had certain influence on the morphological characteristics of Mauve Clusters.With the temperature rised,injury indices increased gradually,and continuous high-temperature stress could make Mauve Clusters wither or even death.When relieving from stress,most plants could recover by themselves.With temperature rised and time prolonged,chlorophyll and carotenoid content were significantly reduced,while MDA and proline content appeared a tendency of slight increase-surging-slight reduction,at the same time,SOD activity rised slowly,and CAT activity firstly increased and then sharply deceased.At the temperature of 35℃ and 40℃,there were little effect on Mauve Clusters cutting seedlings growth.Under the temperature of 45℃ for 1 to 2 days,Mauve Clusters would suffer certain thermal damage,but could restore through self-regulation,when for 3 days,only 45% plants could live through self-regulation,and 55% plant withered or even dead.Under the environment of high temperature,there were significant correlations among injury indices,chlorophyll,MDA,proline,SOD and CAT,which suggested that these physiological indexes could regulate the high temperature damage,and those physiological indexes could be used as heat resistant identification index of Mauve Clusters.