中文摘要：

2015年春,山东滕州某规模化羊场新生羔羊出现严重的腹泻,并且部分羔羊死亡。对该羊场病死羔羊进行剖检后,共分离到4株细菌（分别命名为PM1、PM2、PM3、PM4）。对所分离的细菌进行形态特征、生化试验以及16S rDNA遗传分析,判定该4株菌均为奇异变形杆菌。16SrDNA系统发育及同源性分析显示：4株分离菌与其他11株奇异变形杆菌的同源性为99%以上,其中与中国Hu株、马来西亚PPB3株以及印度BAB-199株位于同一个分支上,且与中国的Hu株的进化关系最近;4株分离菌之间的同源性为100%,判断为同1株菌。细菌生长曲线、药敏试验、毒力试验、毒素测定试验以及游散行为分析结果表明,该分离菌于14h左右达到生长稳定期,且对环丙沙星、左氟沙星最为敏感;对小鼠有强致病性,分离菌培养液的无菌滤液对小鼠无毒性作用;在含0.5%琼脂的LB平板上迁徙速度最快,且在含琼脂1.5%和2.0%的LB平板上呈圆环样周期运动。该研究结果为羊奇异变形杆菌病的防控提供了基础。

英文摘要：

In the spring of 2015, the lambs in a large-scale sheep farm of Tengzhou in Shandong province showed severe diarrhea, and some lambs died. Four bacteria strains（ named PMI,PM2, PM3 ,and PM4） were isolated from the dead lambs after dissection. After identifying with morphological characteristics observation, biochemical tests, and 16S rDNA genetic analysis,all of the 4 i-- solates were Proteus mirabilis. The results of phyl0genetic and homology analysis of 16S rDNA showed that the homology between the 4 isolates and Other 11 Proteus mirabilis strains exceeded 99%,and the 4 isolates were located in the same branch with the reported strains Hu（China）, PPB3（Malaysia） ,and BAB-199（India）,which showed the closest relationship with strain Hu（Chi na）. The homology between the 4 isolates was 100%,which was judged as the same strain. The re- sults of bacterial growth curve,drug sensitivity test,toxicity test,toxin lest,and swarming behav ior showed that the isolate reached the stable stage of growth at about 14 h and was the most sensitive to ciprofloxacin and levofloxacin;It exhibited strong pathogenicity to mice,while its sterile filtrate was non-toxic; It also showed the fastest migration on the LB plate containing 0.5%agar and periodic circular motion on the LB plates containing agar 1.5% and 2%. The results of this study provided technical supports for the prevention and control of sheep Proteus mirabilisdisease.