目的:通过分析不同浓度NaCl胁迫下甘草生长和抗氧化酶活性的变化探讨甘草对盐分胁迫的适应性机理;方法:采用不同浓度的NaCl溶液(配成Hoagland液)处理盆栽一年生甘草,分别于35d、70d和105d取样,测定甘草株高、地上部分鲜、干重、根鲜、干重及甘草叶片SOD、POD、CAT活性,分析各生长指标与抗氧化酶活性的相关性;结果:NaCl胁迫70d和105d,0.6%和0.9%处理组的株高、地上部分鲜、干重及根鲜、干重均显著低于CK,SOD、POD及CAT活性均显著高于CK,经相关性分析得知,SOD、POD及CAT活性与各生长指标均负相关,其中POD活性与各生长指标极显著负相关;结论:甘草对NaCl胁迫的响应有胁迫时间和浓度的依赖性,在遭遇胁迫时,通过改变自身的生长和提高抗氧化酶活性,来提高机体的抗盐能力。
To investigate the effect of salt stress on the growth and antioxidation enzyme activities of Glycyrrhiza ural- ensis. Methods: Annual Glycyrrhiza uralensis was cultivated in the plastic flowerpot irrigated with various concentrations of NaC1 (Hoagland solution) for different duration. The height, fresh/dry weight of shoots, fresh/dry weight of root, SOD, POD and CAT activities were determined 35 d, 70 d and 105 d after treatment. The correlation of the growth indices and antioxidant enzymes activities were analyzed. Results: On 70th and 105th days after the treatment, the height, the shoots fresh/dry weight and the root fresh/dry weight of 0.6 % and 0.9 % treatment groups decreased significantly compared with CK, the SOD, POD and CAT acticity of 0.6 % and 0.9 % treatment groups were extremely higher than those in the control group. The correlation analysis showed that SOD, POD and CAT activities had a negative correlation with growth indices, while POD activity has negative correlation with growth indices. Conclusion: The growth of Glycyrrhiza uralensis depends on NaC1 concentration and the treated duration. When suffered stress, it improves the body salt tolerance by decreasing growth and increasing antioxidation enzyme activities.