中文摘要：

利用ISSR分子标记技术,对采集自安徽省滁州市天然次生林——琅琊山国家森林公园和宣城市人工马尾松纯林——麻姑山林场球孢白僵菌种群的遗传多样性水平和种群遗传结构进行了比较分析。用7个引物分别对2个种群共222株球孢白僵菌菌株进行扩展,共得到58个清晰的扩增位点,其中多态位点56个,多态位点百分率（PPB）为96.55%,Nei＇s基因多样性（H）为0.2993,Shannon信息指数（I）为0.4593,种群间的基因分化系数（Gst）为0.1283,基因流Nm=3.3984;可以看出,2个种群间的基因流较小,遗传分化较大,为12.83%,这可能是由于人为选择和基因流障碍引起的;琅琊山白僵菌群体（PPB=96.55%,H=0.2781,I=0.4299）遗传多样性水平较高;麻姑山白僵菌群体（PPB=93.10%,H=0.2552,I=0.3825）遗传多样性水平较低。比较研究了采集自大别山原始林中球孢白僵菌菌株的遗传多样性（PPB=81.00%,H=0.3187,I=0.4782）,可见生态环境复杂的原始林中球孢白僵菌遗传多样性最高,天然次生林种群次之,人工纯林种群最小。利用Nei’s遗传距离构建琅琊山和麻姑山白僵菌个体间的遗传关系树状图,由UPGMA聚类分析可知,不同采集地的菌株聚为一类。

英文摘要：

ISSR molecular marker technology was adopted to conduct comparison analysis on genetic diversity level and population genetic structure of Beauveria bassiana population in natural secondary forest （Langyashan national forest park） in Chuzhou City of Anhui Province and artificial pure pine forest （Magushan forest farm） in Xuancheng City of Anhui Province.Seven primers were selected to conduct PCR amplification on total 222 strains of B.bassiana in two populations,a total of 58 unique amplified loci were obtained through amplification,the number of polymorphic loci was 56,the percentage of polymorphic loci was 96.55%,Nei＇s genetic diversity was 0.299 3,Shannon information index was 0.459 3,genetic differentiation coefficient among populations （Gst） was 0.128 3,gene flow Nm=3.398 4;the gene flow between the two populations was small,genetic differentiation was relatively large,being 12.83%,this may be caused by human selective pressures and barrier of gene flow;the genetic variation level of B.bassiana populations in Langyashan was relatively high（PPL=96.55%,H=0.278 1,I= 0.429 9）;the genetic variation level of B.bassiana populations in Magushan was relatively low（PPL=93.10%,H=0.255 2,I= 0.382 5）.The genetic diversity of B.bassiana from the primary forest in Dabieshan was studied（PPL=81.00%,H=0.318 7,I= 0.478 2）,indicating that the genetic diversity of B.bassiana populations in Dabieshan with complex ecological environment was the highest,followed by the populations in natural secondary forest,and the genetic diversity in artificial pure pine forest was the lowest.Nei＇s genetic distance was adopted to construct the genetic relationship dendrogram of B.bassiana individuals collected from Langyashan and Magushan,from the cluster analysis of UPGMA,the strains from the same collection places clustered together.