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中文摘要：
目的观察环维黄杨星D（CVB-D）对去甲肾上腺素（NE）诱导心肌细胞损伤的保护作用及对G蛋白偶联受体激酶-2（GRK-2）表达的影响。方法体外培养新生SD大鼠原代心肌细胞,建立NE诱导的心肌细胞损伤模型,以细胞存活率,培养上清液乳酸脱氢酶的（LDH）活性反映心肌细胞损伤。酶联免疫吸附法（ELISA）测定环磷酸腺苷（cAMP）含量,Western blot分析G蛋白偶联受体激酶-2（GRK-2）蛋白表达。结果 NE能降低细胞存活率,提高培养液LDH活性,降低细胞内cAMP含量,增加GRK-2蛋白表达。CVB-D对NE诱导上述指标异常具有明显的改善作用。结论
英文摘要：
Aim To investigate the effects and explore the mechanism of Cyclovirobuxine-D（CVB-D） on expression of GRK-2 in primary culture cardiac myocyte injury induced by norepinephrine（NE）.Methods The primary culture cardiac myocyte injury was reproduced by 20 μmol.L-1.The injury was assayed by the cell survivial rate and LDH activity in medium.cAMP content in the cell lysis solution was measured by ELISA,and the GRK-2 protein expression was detected by Western blot.Results NE significantly decreased the cell survivial rate and cAMP contents in cell lysis solution,and increased the LDH activity in medium and GRK-2 protein expression in cell lysis solution.Pretreated with CVB-D,the abnormal was significantly ameliorated in primary culture cardiac myocyte injury induced by NE.Conclusions CVB-D protects against primary culture cardiac myocyte injury induced by NE,and the mechanism may be involved in associating with depression G-protein coupled receptors signaling pathway via increasing the contents of cAMP and down regulating GRK-2 expression.
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