欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
利用响应面法对小球藻（Chlorella vulgaris）在2L气升式生物反应器中对自养产生物柴油油脂的培养条件进行了优化。首先用Plackett-Burman方法对10个相关影响因素的效应进行评价并筛选出对产油有显著影响的3个因素：KNO3浓度、温度和CO2浓度；再用最陡爬坡实验逼近最大产油区域；最后由中心组合实验及响应面分析确定了影响产油主要因素的最佳条件为：KNO3浓度0．31g／L，温度26．5℃，CO2浓度6．80％，最高产油量达到0．42g／L，比优化前提高了近2倍。优化后，在10L气升式生物反应器中进行了扩大培养。
英文摘要：
Response surface methodology was used to optimize the autotrophic cultivation conditions for lipids production by Chlorella vulgaris in a 2 L airlift photobioreactor. In the first optimization step, a plackett-Burman design was used to evaluate the influence of ten related factors and it was found out that KNO3 concentration, temperature and CO2 concentration influenced lipids production significantly. Subsequently, the path of steepest ascent was used to approach the optimal region of the cultivation conditions. In the third step, KNOaconcentration, temperature and C02concentration were further optimized using central composite designs and response surface analysis and the optimum conditions were that KNO3 concentration was 0. 31 g/L, temperature was 26.5℃ and CO2 concentration was 6.80%. Under optimum conditions, the lipid yield was 0.42g/L and it was increased by two times than that under the conditions before optimized. Chlorella vulgaris was cultured in a 10 L airlift photobioreactor under optimum conditions.
同期刊论文项目
同项目期刊论文
A temperature-shift strategy for efficient arachidonic acid fermentation by Mortierella alpina in ba
Increase of Docosahexaenoic Acid Production by Schizochytrium sp Through Mutagenesis and Enzyme Assa
Cell morphology variations of Klebsiella pneumoniae induced by acetate stress using biomimetic vesic
Efficient chemo - enzymatic synthesis of endomorphin -1 using organic solvent stable proteases to gr
Pervaporation behavior and integrated process for concentrating lignocellulosic ethanol through poly
Improvements of lipase performance in high - viscosity system by immobilization onto a novel kind of
Engineered Bacillus subtilis 168 produces l-malate by heterologous biosynthesis pathway construction
An organic solvent and thermally stable lipase from Burkholderia ambifaria YCJ01: Purification, char
Enhancing the catalytic properties of porcine pancreatic lipase by immobilization on SBA-15 modified
Engineering Klebsiella oxytoca for efficient 2, 3-butanediol production through insertional inactiva
Identification of organic solvent - tolerant lipases from organic solvent - sensitive microorganisms
Efficient cloning and expression of a thermostable nitrile hydratase in Escherichia coli using an au
Efficient dynamic kinetic resolution of arylamines with Pd / layered double - hydroxide - dodecyl su
Enhancing catalytic performance of porcine pancreatic lipase by covalent modification using function
Screening, purification and characterization of a novel cold-active and organic solvent-tolerant lip
Control and optimization of Clostridium tyrobutyricum ATCC 25755 adhesion into fibrous matrix in a f
Enhanced butyric acid tolerance and bioproduction by Clostridium tyrobutyricum immobilized in a fibr
Biodegradation and metabolic pathway of beta-chlorinated aliphatic acid in Bacillus sp. CGMCC no. 41
Engineering Bacillus subtilis for isobutanol production by heterologous Ehrlich pathway construction
Transient CFD modeling of toluene waste gas biodegradation in a gas-liquid-solid three-phase airlift
Purification and characterization of an organic solvent-stable lipase from Pseudomonas stutzeri LC2-
A kinetic and thermodynamic study of the lipase-catalyzed remote resolution of a chiral tertiary alc
Two-stage oxygen supply strategy for enhanced lipase production by Bacillus subtilis based on metabo
Optimization of enzymatic synthesis of L-ascorbyl palmitate by solvent engineering and statistical e
Mesoporous Material SBA-15 Modified by Amino Acid Ionic Liquid To Immobilize Lipase via Ionic Bondin
Synthesis and release studies of microalgal oil-containing microcapsules prepared by complex coacerv
Kinetic characterization for hemicellulose hydrolysis of corn stover in a dilute acid cycle spray fl
Disruption of Chlorella vulgaris Cells for the Release of Biodiesel-Producing Lipids: A Comparison o
Extreme-thermophilic biohydrogen production from lignocellulosic bioethanol distillery wastewater wi
Enhancement of Fumaric Acid Production by Rhizopus oryzae Using a Two-stage Dissolved Oxygen Control
Enhancement of docosahexaenoic acid production by Schizochytrium sp. using a two-stage oxygen supply
Elimination of carbon catabolite repression in Klebsiella oxytoca for efficient 2,3-butanediol produ
Improved 2-methyl-1-propanol production in an engineered Bacillus subtilis by constructing inducible
Higher-level production of ascomycin (FK520) by Streptomyces hygroscopicus var. ascomyceticus irradi
Effect of CO(2) supply conditions on lipid production of Chlorella vulgaris from enzymatic hydrolysa
Development of a stepwise aeration control strategy for efficient docosahexaenoic acid production by
期刊信息
位置：