中文摘要：

为了解宁夏地区奶牛乳房炎金黄色葡萄球菌携带氟喹诺酮类药物的耐药基因的情况,采用PCR对临床分离鉴定的220株金黄色葡萄球菌的gyrA、norA和grlA基因进行了检测,并对PCR产物进行测序和分析。结果显示,gyrA基因、grlA基因和norA基因的检出率分别为80%、78.64%和78.18%。测序结果显示,grlA、norA基因的同源性为99%,gyrA基因的同源性为100%。这表明在宁夏地区gyrA、norA和grlA基因已广泛存在。

英文摘要：

In order to understand the condition of carrying fluoroquinolone resistant gene of Staphylococcus aureus isolated from bovine mastitis milk samples in dairy farms in Ningxia region,the gyrA,norA and grlA drug-resistant genes of 220 strains of Staphylococcusaureus were detected by PCR in this study,and PCR products were sequenced and analyzed.The results showed that the detection rate of gyrA,norA and grlA were 80%,78.18% and 78.64% respectively.The sequencing results showed that the homologies of grlA and gyrA were 99%,and the homology of norA was 100%.This suggests that gyrA,norA,grlA genes are widely existed in Ningxia region.