以干柑橘皮为原料,采用酸提醇沉法对其果胶进行提取。通过单因素、响应面分析法等设计系统研究料液比、提取温度、时间、pH值等影响果胶得率的因素,对果胶提取条件进行优化和显著性分析,从而确定果胶提取的最佳条件为液料比19.7:1、提取温度87.5、提取时间84min、pH1.51。在最优条件下提取果胶的得率为7.294%,与预期值(7.316%)相差不大,因而优化的组合可用于指导产业化制备果胶。
Acid extraction followed by ethanol precipitation was used to extract pectin from citrus peel in the present work.Four factors that affect the extraction yield of pectin including material-to-liquid ratio,temperature,extraction time and pH were systematically investigated by one-factor-at-a-time method and response surface analysis.Further,the four factors were optimized and their significance was analyzed.As a result,the optimal conditions for pectin extraction were determined as follows: material-to-liquid ratio 19.7:1,temperature 87.5 ℃,extraction time 84 min,and pH 1.51.Under these conditions,the actual pectin yield was 7.294%,which was close to the predicted value of 7.316%.Thus,the optimized process can provide useful guidance for industrial pectin preparation.