中文摘要：

以4个新疆野苹果株系为试材，利用CTAB法提取DNA,对影响SSR—PCR扩增结果的主要因子设计了多梯度的优化试验。结果表明：新疆野苹果SSR-PCR反应体系（25μL）中含ToqDNA聚合酶0-5U、模板DNA5．0ng、dNTPs0．2mmol／L、引物0．2μmol／L、Mg2＋1．0mmol／L、退火温度为60％时效果最佳。最佳扩增程序为：94℃预变性2min，94℃变性30s，65℃退火1min，72~C~伸lmin，4个循环；94cc变性30s，6d℃退火1min，72℃延伸1min，30个循环；72％延伸5min，4℃保存。利用此反应体系对30份新疆野苹果进行SSR-PCR扩增和电泳检测，扩增谱带清晰且多态性较好，表明该体系适用于新疆野苹果的基因连锁图谱构建和QTL定位。

英文摘要：

DNA of four Malus sieversii accessions were distilled by CTAB.An optimal experimental design was employed to evaluate five factors （template DNA,Mg2＋,dNTPs, Taq DNA polymerase and primer） at five different concentrations.The results indicated that the optimal SSR-PCR reaction system of terminal volume 25 ILL consisted of 0.5 U Taq DNA polymerase,0.2 txmol/L primers,5 ng template DNA,0.2 retool/ L dNTP and 1.0 mmol/L Mg2~.The suitable amplification temperature profiles were as following：2 vain at 9422 for pre-denaturing, followed by 4 cycles of 30 s at 9422 for denaturing,1 rain at 6522 for anncaling,1 rain at 7222 for extending,then followed by 30 cycles of 30 s at 9422 for denaturing,1 min at 6022 for annealing,1 min at 7222 for extending, and finally kept the reaction mixture at 422 after a final extension step of 7222 for 5 min.Using the above optimal SSR-PCR reaction system,SSR fragments of 30 Malus sieversii accessions were obtained.The clear and abundant polymorphism indicated this reaction system was suitable for construction gene linkage map and QTL mapping.