中文摘要：

以二茂铁，Gly，Asp和Boc-Arg（NO2）-OH为原料，采用液相合成法，以苯并三唑-1-四甲基六氟磷酸酯（HBTU）和1-羟基苯并三唑（HOBt）为缩合剂合成了二茂铁-肽化合物Ferrocene-Arg（NO2）-Gly—Asp（OMe）-OMe（5），其收率为38．7％，并对化合物5进行了表征．采用荧光光谱及紫外-可见吸收光谱研究了近生理条件下化合物5与牛血清白蛋白（BSA）的相互作用．实验结果表明化合物5与BSA间相互作用为静态猝灭过程，反应结合位点数为0．856，结合常数为1．75×10^3L·mol^-1；并依据能量转移理论确定了其与BSA相互作用的最近距离为2．14nm．

英文摘要：

The ferrocene conjugated Fc-Arg（NO2）-Gly-Asp（OMe）-OMe （5） was synthesized from ferrocene, Gly, Asp and Boc-Arg（NO2）-OH by the method of solution synthesis with a yield of 38：7%, and characterized by ^1H NMR, IR and ESI-MS. Fluorescence spectrum and UV-visible absorption spectrum were used to investigate the interaction between compound $ and bovine serum albumin （BSA）. The experimental results indicated that the interaction involved a static quenching with a conformational change of BSA. The number of binding site n and equilibrium constant Were 0.856 and 1.75×10^3 L·mol^-1, respectively. Based on the energy transfer theory, the binding distance between compound 5 and BSA was 2.14 nm.