目的:建立人参多糖含量测定方法。方法:以人参精制多糖测得人参多糖对葡萄糖的换算因子,以苯酚-硫酸分光光度法测定人参多糖的含量。结果:供试液在2h内显色稳定,重现性好,平均回收率为99.8%,RSD为2.57%。人参多糖含量为28.56%,RSD为1.81%。结论:该方法简便,快速,测定结果客观,准确。
Objective:To establish a method for content determination of polysaccharides in the root of Panax ginseng.Method:The conversion coefficient of ginseng polysaccharide to glucose was obtained by refined polysaccharides,and then the content of crude polysaccharides in the root of Panax ginseng was determined by the method of phenol-sulferic acid.Result:the color of the treated solution showed good stabilization within 2h and the average value of the recovery for polysaccharide measurement was 99.8% with 2.57% of RSD.The contents of polysaccharide in the root of Panax ginseng was 28.56% with 1.81% of RSD.Conclusion :The method was simple,rapid and accurate.