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中文摘要：
目的以油菜原蜜为研究对象,通过测定其中果糖、葡萄糖、蔗糖和麦芽糖的含量评价我国大宗原蜜的品质。方法采用高效液相色谱示差折光法测定我国10个省份共36种油菜原蜜样品中果糖、葡萄糖、蔗糖和麦芽糖的含量。结果用液相色谱方法测定蜂蜜中的果糖、葡萄糖、蔗糖和麦芽糖,最低检测限分别为0.079 g/100 g、0.018 g/100 g、0.037 g/100 g及0.094 g/100 g,定量检测限分别为0.263 g/100 g、0.061 g/100 g、0.124 g/100 g及0.314 g/100 g。36种油菜原蜜中果糖、葡萄糖、蔗糖和麦芽糖的含量范围分别为26.00~38.16g/100 g、23.65~39.68 g/100 g、0.14~0.62 g/100 g及1.09~3.10 g/100 g。结论油菜原蜜样品中的蔗糖含量都符合我国蜂蜜国家标准,但其中31.8%的样品中葡萄糖和果糖总量低于国家标准。目前,我国大宗蜂蜜不存在人为掺入蔗糖的问题。
英文摘要：
ABSTRACT:Objective To evaluate the quality of Chinese honey by the content of fructose, glucose, sucrose and maltose in rape honey. Methods The content of fructose, glucose, sucrose and maltose of 36 kinds of rape honey were analyzed by high performance liquid chromatography method of refractive index (HPLC-RI).Results The content of fructose, glucose, sucrose and maltose of rape honey were 26.00~38.16 g/100 g, 23.65~39.68 g/100 g, 0.14~0.62 g/100 g and 1.09~3.10 g/100 g, respectively. The lowest detection limit (LOD) were 0.079 g/100 g, 0.018 g/100 g, 0.037 g/100 g and 0.094 g/100 g, respectively. The quantitative detection limit (LOQ) were 0.263 g/100 g, 0.061 g/100 g, 0.124 g/100 g and 0.314 g/100 g, respectively. Conclusion The sucrose content of all rape honey conforms to the national standard, but total content of fructose and glucose of 31.8% rape honey is lower than national standard. At present, there is no artificial mixed with sucrose in Chinese honey.
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