以黄酮提取率为指标,采用超声辅助法研究了芒草中黄酮类化合物的提取工艺。最佳提取条件为:乙醇浓度70%、料液比1∶30、提取温度70℃、提取时间40 min、提取级数3次,在此条件下测得南荻中总黄酮含量为7.24 mg/g,并在此条件下测定了芒草10个品系中总黄酮含量为4.16~7.84 mg/g,且在不同品系间的差异均达到极显著水平。采用气质联用技术鉴定奇岗六倍体、‘GMG121’、芒杂中的挥发油成分,从中共鉴定出28种成分。本研究对芒属植物的遗传育种以及开发和综合利用具有重要意义。
An ultrasonic assisted method was used to study the optimum extraction of flavonoids in Miscanthus. The determined optimal extraction conditions of flavonoids were as follows: 70%ethanol concentration,1∶30 ratio of raw material to liquid,70℃ extraction temperature,40 min extraction time,and three extract series,respectively. Under these conditions,total flavonoid content of M. lutarioriparius was 7.24 mg / g. The experimental yields of flavonoids from various kinds of M. sinensis were significantly different,ranging from 4.16 mg / g to 7. 84 mg / g. The volatile oil components in Miscanthus x giganteus( hexaploid),‘GMG121',and M. sinensis hybrid were determined by GC / MS, with 28 different components identified. This study provides useful information on genetic breeding, which has great significance for the development and utilization of M. sinensis.