中文摘要：

细胞色素P450基因在电子传递链、次生代谢物质合成和对外源化学药物毒性降解中发挥着重要作用,为了深入了解该基因在甘蔗中的功能,通过RT-PCR扩增获得甘蔗细胞色素P450还原酶基因的cDNA全长序列,命名为ScCPR450（Gen Bank Accession Number：KR864841）.该基因全长999 bp,含有744 bp的完整开放阅读框,编码247个氨基酸.亚细胞定位结果显示,ScCPR450蛋白分布于细胞质中,与生物信息学预测结果相符.q RT-PCR表达分析表明,该基因在甘蔗中组成型表达,但有组织特异性,芽中表达量最高,其次是叶,而皮中表达量最低.在脱落酸（ABA）、水杨酸（SA）、茉莉酸甲酯（Me JA）、聚乙二醇（PEG）和氯化铜（CuCl_2）胁迫诱导过程中,该基因的表达量呈现不同变化模式,其中SA胁迫6 h下,ScCPR450基因的表达量最高,约为对照的12.21倍;在PEG胁迫下,ScCPR450基因的表达量上调且表达量稳定,推测ScCPR450基因在甘蔗响应生物和非生物胁迫中发挥一定的作用.本研究可为该基因家族其它成员的克隆以及深入解析该基因的功能特性奠定基础,进而为基于基因工程技术对甘蔗品种进行定向改良提供基因资源.

英文摘要：

Cytochrome P450 gene plays an important role in the electron transport chain, secondary metabolite synthesis and degradation of exogenous chemical drug toxicity. In order to understand the function of this gene in sugarcane, this study used the EST CF576130.1 with homologs to a sugarcane cytochrome P450 reductase gene as a probe, performed electriccloning technology combined with RT-PCR amplification, and obtained the full length cDNA sequence of sugarcane cytochrome P450 reductase gene termed as ScCPR450（Gen Bank Accession Number：KR864841）. Bioinformatics analysis revealed that the length of ScCPR450 was 999 bp, which contained a complete open reading frame with a length of 744 bp, encoding 247 amino acid residues. Subcellular localization showed that ScCPR450 was distributed in the cytoplasm which was in consistence with the bioinformatics prediction result. The q RT-PCR analysis revealed that ScCPR450 was constitutively expressed and had tissuespecificexpression in sugarcane, with the highest expression level in the bud, followed by the leaf and the lowest expression in the epidemis. At the same time, the expression patterns of ScCPR450 were different after challenging with ABA, SA, Me JA, PEG and Cu Cl_2. Under the SA stress for 6 h, the expression level of ScCPR450 was the highest, about 12.21 times more than that in control. Under the stress of PEG, the transcripts of ScCPR450 raised and remained stable. The results suggested that ScCPR450 played a role in response to bioticand abioticstresses in sugarcane. This study lays a certain foundation for the cloning of other members of CPR family genes and also for further analysis of the gene functional characteristics, which ultimately provides gene resource for the directional improvementment of sugarcane varieties through geneticengineering.