采用玉米籽粒离体培养的方法研究了不同培养方式对玉米籽粒发育的影响,并对籽粒体积、粒重、可溶性糖、淀粉、蛋白质含量以及呼吸的变化动态进行了测定。结果表明,采用籽粒:穗轴为3:10的培养方式较好。离体籽粒的发育与大田玉米相同,该技术可以广泛应用于玉米生理研究。
Maize kernel development of different models was studied under culture in vitro. Kernel volume, kernel weight, content of soluble sugar, starch, protein and kernel respiration were also measured. The result was showed that the model of kernel : cob as 3 : 10 was the best. Kernel development under cultured in vitro was the same as the field grow maize. This culture technique can be generally used in maize physiology study.