中文摘要：

以龙眼（Dimocarpus longanLour.）为试材,应用同源克隆和RACE方法从花芽中获得了调控蛋白14-3-3的全长cDNA序列,GenBank登录号为FJ479618（GI：218202931）。该cDNA全长1121bp,包括一个783bp的开放阅读框,编码261个氨基酸,序列比较分析显示14-3-3cDNA具有较高的保守性。半定量RT-PCR分析结果表明,14-3-3mRNA在龙眼叶芽、叶片、花芽和成熟的花中都有表达,但在花芽中表达量最大。构建了pET14-3-3原核表达系统,将14-3-3全长cDNA在大肠杆菌中表达,获得一个分子量约为34kD的可溶性融合蛋白,经Western blotting验证,该蛋白为14-3-3蛋白。

英文摘要：

Full length cDNA of 14-3-3 gene, encoding a plant regulatory factor, was isolated from longan （Dimocarpus longan Lout. ） using homology-based cloning and RACE methods. The accession number of the gene in GenBanks is FJ479618 （GI： 218202931 ）. The full length eDNA is of I 121 bp, with a 783 bp open reading frame encoding 261 amino acids. Homology analysis showed that the longan 14-3-3 eDNA has high consensus regions. Semi-quantitative RT-PCR analysis detected the expression of 14-3-3 gene in longan foliar buds, leaves, mature flowers, and most up-regulated in flower buds. The prokaryotic expression vector was constructed and was expressed in E. coli; and a recombinant protein with a molecular weight of 34 kD was obtained by SDS-PAGE analysis. Western blotting confirmed the protein was 14-3-3 protein.