中文摘要：

反向抄写的质的 PCR (RT-qPCR ) 被用来在硫的抄写层次分析变化在硫上成年或铁的 Acidithiobacillus ferrooxidans ATCC 23270 的新陈代谢相关的 periplasmic 蛋白质基因。有比在铁的硫酸盐上在元素的硫上种的显然更高的丰富的七 periplasmic 蛋白质被帮助矩阵的激光解吸附作用电离 time-of-flight 团 spectrometry (MALDI-TOF-MS ) 识别。由 RT-qPCR 的相应基因的表示分析证明所有那些基因的组成的表示是更起来调整的比在上成年的那些在硫上成长铁(> 10 文件夹) 。由 RT-qPCR 的识别 periplasmic 蛋白质的相应基因上的学习证实了二尺寸的胶化电气泳动的结果，显示他们可能与在 A 的硫新陈代谢是相关的。ferrooxidans。

英文摘要：

Reverse-transcription qualitative PCR（RT-qPCR）was used to analyze the changes in transcription levels of the sulfur metabolism-related periplasmic protein genes of Acidithiobacillus ferrooxidans ATCC 23270 grown on sulfur or ferrous.Seven periplasmic proteins with apparently higher abundance grown on elemental sulfur than on ferrous sulfate were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry（MALDI-TOF-MS）.Expression analysis of the corresponding genes by RT-qPCR shows that the constitutive expression of all those genes are more up-regulated grown on sulfur than those grown on ferrous（10 folder）.Study on the corresponding genes of the identified periplasmic proteins by RT-qPCR confirmed the results of two-dimensioned gel electrophoresis,indicating they may be related with sulfur metabolism in A.ferrooxidans.