种子休眠是在谷物的一个重要农学的特点。用深休眠(N22 ) ,中等休眠(ZH11 ) ,并且非休眠(G46B ) 大米栽培变种,我们相关在种子开发和吸收期间与 abscisic 酸(骆驼毛的织物) 和赤霉素(GA ) 新陈代谢基因表示侧面和植物激素层次播种休眠显型。骆驼毛的织物和在种子开发期间满意的 GA 的时间功课分析证明 N22 让一件高骆驼毛的织物在早、中间的种子铺平发展阶段,当在晚发展的舞台它衰退了到 ZH11 的水平时;然而,它的 ABA/GA 比率在整个种子开发在高水平维持了。由对比,尽管在早发展的舞台它的骆驼毛的织物水平接近了 ZH11 的, G46B 在种子开发期间有最低骆驼毛的织物内容,并且它的 ABA/GA 比率在在一样的晚发展的舞台达到顶点 ZH11 的水平。与 N22 和 G46B 相比, ZH11 在种子开发期间有平、中等的骆驼毛的织物水平,它的 ABA/GA 比率在中间的发展阶段达到顶点。而且,有高 ABA/GA 比率的种子发展时间点也越过三栽培变种在骆驼毛的织物和 GA 新陈代谢小径为关键基因有相对高的抄本层次。这些显示强加胚胎的休眠在晚发展的阶段前被导致了并且被 ABA/GA 比率决定。类似的分析在种子吸收期间证明那件骆驼毛的织物为三栽培变种在不同的度被综合。另外,为未经触动的成熟种子的水举起试金建议水能为所有三栽培变种通过外壳障碍渗入进种子胚胎;然而,所有三栽培变种在他们的外壳由 flavans 的存在的染色香草醛的陈述语气显示出不同颜色,它是为强加外壳的休眠负责的休眠抑制混合物。
Seed dormancy is an important agronomic trait in cereals. Using deep dormant (N22), medium dormant (ZH11), and non-dormant (G46B) rice cultivars, we correlated seed dormancy phenotypes with abscisic acid (ABA) and gibberellin (GA) metabolism gene expression profiles and phytohormone levels during seed development and imbibition. A time course analysis of ABA and GA content during seed development showed that N22 had a high ABA level at early and middle seed developmental stages, while at late developmental stage it declined to the level of ZHll; however, its ABA/GA ratio maintained at a high level throughout seed development. By contrast, G46B had the lowest ABA content during seed development though at early developmental stage its ABA level was close to that of ZH11, and its ABA/GA ratio peaked at late developmental stage that was at the same level of ZHll. Compared with N22 and G46B, ZH11 had an even and medium ABA level during seed development and its ABA/GA ratio peaked at the middle developmental stage. Moreover, the seed development time-point having high ABA/GA ratio also had relatively high transcript levels for key genes in ABA and GA metabolism pathways across three cultivars. These indicated that the embryo-imposed dormancy has been induced before the late developmental stage and is determined by ABA/GA ratio. A similar analysis during seed imbibition showed that ABA was synthesized in different degrees for the three cultivars. In addition, water uptake assay for intact mature seeds suggested that water could permeate through husk barrier into seed embryo for all three cultivars; however, all three cultivars showed distinct colors by vanillin-staining indicative of the existence of flavans in their husks, which are dormancy inhibition compounds responsible for the husk-imposed dormancy.