中文摘要：

毕赤酵母经过20多年的发展,在实验室和工业规模都取得了广泛的应用。文中从工业技术应用的角度,综述了近年来毕赤酵母在蛋白表达、遗传操作方法以及化学品生产方面取得的成果,同时对毕赤酵母系统存在的问题以及未来的研究方向进行了分析和展望。

英文摘要：

With more than 20 years of development, Pichia pastoris system has been extensively used both on a lab and industrial scale. This review outlines the progress made on P. pastoris from aspects of protein expression, molecular engineering tools and methods, and biochemical production. This review also provides perspectives on the current challenges and future directions of this important system.