中文摘要：

探讨白黎芦醇（RSV）减轻Ⅱ型糖尿病（T2DM）小鼠心肌缺血／再灌注损伤（MI／RI）的作用及其机制。方法：采用喂养高脂饮食结合小剂量链脲佐菌素（STZ）建立T2DM小鼠模型。造模成功后立即给予RSV（10mg／kg）每日1次灌胃，连续3周。实验分为正常假手术组、正常手术对照组、DM假手术组、DM手术对照组、RSV组、CpC组，每组20只。手术方式采用心脏冠状动脉左前降支结扎30min、再灌注3h或24h。抑制剂组于术前1h腹腔注射CompoundC（20mg／kg）。用TI＇C染色法检测心肌梗死（MI）面积，TUNEL法检测心肌细胞凋亡，ELISA法检测caspase-3活性、血浆脂联素（APN）水平，Westernblot法检测AMPK、p-AMPK及脂肪组织APN的含量。结果：喂养高脂饮食结合小剂量STZ能够成功建立T2DM小鼠模型。与DM手术对照组相比，RSV饲喂能够减轻T2DM小鼠MI／RI，减小MI面积、减少心肌细胞凋亡（P〈0．01），降低caspase-3的活性（P〈0．05）。RSV还能够上调脂肪组织APN表达，逆转T2DM小鼠低APN血症（P〈0．01）。AMPK抑制剂CompoundC可显著减弱RSV的心肌保护作用（P〈0．05）。结论：RsV可通过逆转T2DM小鼠的低脂联素血症，在MI／RI时发挥对心肌的保护作用。

英文摘要：

T2DM was induced by a high-fat diet （HD） plus low-dose i.p. streptozotocin （STZ） injection. Mice were treated with 10 mg/kg RSV daily by intragastric administration for 3 weeks after acknowledgement of T2DM. Mice were divided into six groups： sham group, I/R group, T2DM sham group, T2DM ＋ I/R group, RSV group, and CpC group. After 30 rain ischemia by slip-knot ligature of the left anterior descending coronary artery, myocardium was reperfused for 3 h after knot release （for apoptosis by TUNEL, caspase-3 activity by ELISA） or 24 h （for infarct size determination by TYC staining）. At 1 h before MI/RI, com- pound C （an AMPK inhibitor） was administered i.p. （20 mg/kg）. RESULTS： HD feeding plus low- dose STZ injection successfully induced T2DM. RSV alleviated MI/RI in diabetic mice as evidenced by decreased infarct size, cardiomyocyte apoptosis （ P 〈 0.01 ） and caspase-3 activity （ P 〈 0. 05 ）. RSV treatment also improved APN level both in plasma and adipose tissue in diabetic mice （ all P 〈 0.01 ）. Conversely, administration of AMPK inhibitor compound C significantly attenuated the cardioprotective