中文摘要：

目的建立交流箱大鼠心理应激模型,研究情绪应激对大鼠咀嚼肌机械疼痛阈值的影响。方法50只SD大鼠,随机分为5组：空白对照组、药物对照组、盐水对照组、足部电击组和情绪应激组,实验时后4组大鼠均同处在交流箱内,同条件饲养。参照Ren方法,根据von Frey纤维粗细和动物痛觉反应次数,确定咬肌和颞肌疼痛分值。结果1）应激后大鼠咬肌和颞肌疼痛阈值出现变化,在7 d的时候出现最高峰,12-14 d的时候达到稳定状态,但与空白对照组相比疼痛阈值还是降低了。2）应激后药物对照组疼痛阈值变化趋势同情绪应激组,但与情绪应激组相比疼痛阈值要高。结论情绪应激可以导致咬肌和颞肌痛觉敏感,抗抑郁药物可以降低应激导致的疼痛敏感度。

英文摘要：

Objective To study the influence of emotion stress on mechanical hyperalgesia of masseter muscles in rats through the equipment of communication box. Methods 50 Sprague-Dawley rats were randomly divided into 5 groups： Control group, emtion stress （ES） group, drug control group, saline treated control group and footshock （FS） group, 10 rats each group. Footshock group only induced emotion stress and was not concerned with the result of the experiment. The last four groups of the rats were placed in communication box and bred under the same conditions during the experiment time. According to Rens＇ method, yon Frey filaments were used to assess the muscle mechanical threshold, head withdrawal, leg raising and crying were observed as pain action. Results 1） During the adaptive period of 7 days before the emotion stress experiment, mechanical pain values of bilateral masseter muscles were descent and were stabilized in the 5 th to 7 th day. In emotion stress period, hyperalgesia of emotion stress group was induced in bilateral masseter muscles, and the peak time was the 7 th day, then it alleviated in the following days and was stabilized in the 12 th to 14 th day, but its pain threshold was lower than blank control group. 2）During the emotion stress period, the change of drug control group was similiar to stress group but its pain threshold was higher. Conclusion It is suggested that emotion stress can lead to the hyperalgesia of masseter muscles and antidepressant drug can lower the hyperalgsia resulted of emotion stress.