中文摘要：

目的探讨正畸力对牙周组织胰岛素样生长因子（IGF）表达的影响，从体内实验角度阐明雌激素和应力对局部骨改建的调控途径和机制。方法建立在Wistar雌性大鼠动情周期不同阶段给予正畸加力的实验模型，采用核酸原位杂交技术检测雌性大鼠动情周期不同阶段牙周组织中IGFmRNA的表达变化。采用SPSS11．0软件包，单因素方差分析法（onewavANOVA）比较动情周期同一阶段加力实验组与对照组差异，Student—Newman—Keuls（S—N—K）法进行同一组内动情周期不同阶段各亚组间的两两比较。结果正畸牙移动使处于动情周期不同阶段的雌性大鼠牙周组织中的IGFmRNA含量增加，其中IGF—ImRNA的表达在动情期最低，而动情前期最高（P〈0．05）；而IGF—IImRNA的表达在动情周期中没有明显规律。结论IGF—I不仅可以在应力反应中由骨形成细胞在局部合成，而且还与动情周期的全身激素状态有关，而IGF-Ⅱ则是对应力发生反应的一种局部生长因子。

英文摘要：

Objective To elucidate the regulatory bio-mechanism of estrogen and local stress on the bone remodeling activities in vivo. Methods A Wistar rat experimental model was established to apply orthodontic activation during different stages of estrous cycle. In situ hybridization was used to detect the variation of the insulin-like growth factors （IGF） mRNA expression in the periodontal tissues. The data of each group was analyzed with SPSS 11.0 software package for Student-Newman-Keuls （S-N-K） test and one way ANOVA test. Results Both IGF- I mRNA and IGF-Ⅱ mRNA level expressed in the periodontal tissues were increased by orthodontic activation during various stages of the estrous cycle. But a similar pattern emerged that the IGF- I mRNA level was the lowest in the estrous stage, whereas its level was the highest in the proestrus stage （P〈0.05）. No rhythm of IGF-Ⅱ mRNA expression found during the estrous cycle. Conclusion It was concluded that local mechanical stimulation directly enhanced the IGF expression. In contrast to the IGF-Ⅱ, IGF-I was also influenced by the sex hormone rhythm during the estrous cycle.