中文摘要：

生长拘捕特定的基因 7 (GAS7 ) ， growth-arrest-specific 家庭的一个成员，编码三蛋白质 isoforms (GAS7A， GAS7B，和 GAS7C ) 并且作为肿瘤 suppressor 基因在肺癌症起一个潜在的作用。在现在的学习，我们在癌(HCC ) 房间与正常的肝相比衬里的 hepatocellular 发现了 GAS7C mRNA 和蛋白质的低内长的表情房间，和那有在与 oxaliplatin 对待的 HCC 房间的 GAS7C 表示的不同增加。CCK8， apoptosis，和 Transwell 迁居试金显示出那房间增长和 HepG2 的活动性， MHCC-97 H 房间被 oxaliplatin 禁止，当 apoptosis 被增加时。有趣地，西方的污点分析证明有 oxaliplatin 的治疗增加了 GAS7C 和 N 黄蜂蛋白质层次并且减少蛋白质的层次在 fibronectin/integrin/FAK 小径包含了，例如 FAK，在两根 HCC 房间线。另外，宫外地， overexpressed GAS7C 显然禁止了房间增长和房间活动性。流动 cytometry 结果证明 GAS7C 的 overexpression 导致了 HepG2 和 MHCC-97 H 房间的 apoptosis。我们进一步由 q-PCR 和西方的污点分析证实了在 GAS7C 和 N-WASP/FAK/F-actin 小径之间的关联在 GAS7C-overexpressing HepG2 和 MHCC-97 H 房间。GAS7C 的抑制实质地颠倒了 oxaliplatin 的反癌症效果并且堵住了 N-WASP/FAK/F-actin 小径的活动。一起拿，我们的结果证明 oxaliplatin 由起来调整的 GAS7C 禁止 HCC 房间增长和迁居能力并且激活 N-WASP/FAK/F-actin 小径。

英文摘要：

The growth arrest-specific gene 7 （GAST）, a member of the growth-arrest-specific family, encodes three protein isoforms （GAS7A, GAS7B, and GAS7C） and plays a potential role in lung cancer as a tumor suppressor gene. In the present study, we found low endogenous expressions of GAS7C mRNA and protein in hepatocellular carcinoma （HCC） cell lines compared with normal liver cells, and that there was a distinct increase of GAS7C expression in HCC cells treated with oxaliplatin. CCK8, apoptosis, and Transwell migration assays showed that cell proliferation and motility of HepG2 and MHCC-97 H cells were inhibited by oxaliplatin, while apoptosis was increased. Interestingly, western blot analysis showed that treatment with oxaliplatin increased GAS7C and N-WASP protein levels and decreased the levels of proteins involved in the fibronectin/integrin/ FAK pathway, such as FAK, in both HCC cell lines. In addition, ectopically overexpressed GAS7C obviously inhibited cell proliferation and cell motility. Flow cytometry results showed that overex- pression of GAS7C induced apoptosis of HepG2 and MHCC-97 H cells. We further confirmed the correlation between GAS7C and the N-WASP/FAK/F-actin pathway by q-PCR and western blot analysis of in GAS7C-overexpressing HepG2 and MHCC-97 H cells. Inhibition of GAS7C substantially reversed the anti-cancer effect of oxaliplatin and blocked the activity of the N-WASP/FAK/F-actin pathway. Taken together, our results showed that oxaliplatin inhibits HCC cell proliferation and migration ability by up-regulating GAS7C and activating the N-WASP/FAK/F-actin pathway.