中文摘要：

采用PMA和ox–LDL构建THP–1巨噬细胞源性泡沫细胞模型，观察不同浓度(5、10、15nmol/L)仙人掌多糖(ODP–Ia)对泡沫细胞内脂质、胆固醇蓄积以及介导胆固醇流出的ABCA1、ABCG1、SR–BI基因表达的影响。结果显示：THP–1巨噬细胞泡沫化后胞内蓄积了大量脂质，高剂量(15nmol/L)ODP–Ia能够显著减少胞内脂质、胆固醇蓄积，并增强ABCA1、ABCG1、SR–BImRNA及蛋白表达(与单独ox–LDL处理组比较，P<0.05)，但效果均次于阳性对照依折麦布组(P<0.05)，低、中剂量(5、10nmol/L)ODP–Ia作用效果均不显著。本研究结果表明，ODP–Ia可通过增强ABCA1、ABCG1、SR–BI基因表达来减少胞内胆固醇蓄积。

英文摘要：

In this study,THP–1cells were induced into macrophages and THP–1macrophage–derived foam cell by phorbolmyristate acetate(PMA)and oxidized low–density lipoprotein(ox–LDL),and then treated with different concentrations(5,10,15nmol/L)of ODP–Ia,to investigate the beneficial effects of ODP–Ia on the cholesterol accumulation in the THP–1macrophage derived foam cell.Meanwhile,the mRNA expression and protein levels of ATP binding cassette transporter ABCA1,ATP binding cassette transporter ABCG1and scavenger receptor class B type I(SR–BI)that mediate cholesterol efflux were observed.The result showed that,the lipid content and the cholesterol content were increased significantly after the THP–1cells were induced into the THP–1macrophage derived foam cell,and then were significantly decreased after treating with15nmol/L ODP–Ia.The data also demonstrated that,compared with the ox–LDL treated group,the high dose group(15nmol/L ODP–Ia treated group)showed significantly increase in the mRNA and protein expressions of ABCA1,ABCG1and SR–BI(P<0.05).However,the high dose group was inferior to the ezetimibe treated group(P<0.05).And there were no significant differences among the low dose(5nmol/L)group,the medium dose(10nmol/L)group and the ox–LDL treated group.In summary,ODP–Ia promoted cholesterol efflux ofTHP–1macrophage derived fo am cell by up–regulation of ABCA1,ABCG1and SR–BI.