中文摘要：

目的 利用量子点双色探针同时标记乳腺癌细胞膜上的人表皮生长因子受体2（HER2）及细胞外基质中的Ⅳ型胶原并进行定量,为乳腺癌细胞侵袭转移研究提供直接的形态学证据及数据支持.方法 应用量子点免疫荧光双标法检测54例乳腺癌组织中HER2与Ⅳ型胶原蛋白的共表达并进行定量分析其与各临床病理参数的相关性.结果 HER2与Ⅳ型胶原蛋白的表达呈负相关（r=-0.980,P＜0.05）;HER2和Ⅳ型胶原蛋白的表达与患者年龄、绝经状况及激素受体状态无明显相关性（P＞0.05）,而与腋窝淋巴结转移、TNM分期及无病生存期有明显相关性,差异有统计学意义（P＜0.05）.结论 利用量子点免疫荧光双标法同时检测HER2和Ⅳ型胶原蛋白的表达可为乳腺癌的浸润转移倾向和预后提供依据.

英文摘要：

Objective By using quantum dots-based double-color imaging method to simultaneously label human epidermal growth factor 2 （HER2） on breast cancer cell membrane and the type Ⅳ collagen in the extracellular matrix.To monitor the degree of malignancy of breast cancer and the invasive and metastatic potential.Methods The co-expressions of HER2 and the type Ⅳ collagen in breast cancer of 54 cases were detected and quantified by quantum dots-based double-labeling immunofluorescent histochemistry,and its correlation with clinical pathology parameters and prognosis were analyzed.Results With the increasing HER2 expression level,a progressive decrease in collagen Ⅳ around the cancer nest,the expression of HER2 and the type Ⅳ collagen value was negatively correlated （r =-0.980,P ＜ 0.05） ; the expression of HER2 and collagen Ⅳ was associated with lymph note metastasis,pathological stage （TNM） and disease-free survival（P ＜ 0.05）.The expression of HER2 and the type Ⅳ collagen between different age groups,menopause and estrogen receptor （ER） and progesterone receptor （PR） were not significantly different （P ＞ 0.05）.Conclusions Quantum dots-based double-color imaging method provides direct observable evidence to support the degradation of HER2 and the type Ⅳ collagen expression,which may help in determining the degree of malignancy and evaluating prognosis.