中文摘要：

以高胡萝卜素自交系P50006和HCM A.C.为亲本构建的F2群体为作图群体,对胡萝卜中α-胡萝卜素、α-胡萝卜素、总胡萝卜素和番茄红素含量进行QTL定位及遗传分析。结果表明,α、β-胡萝卜素、总胡萝卜素和番茄红素含量的广义遗传力分别为0.75、0.50、0.31和0.93。遗传图谱包含91个SRAP（Sequence-related amplified polymorphism）标记,分布于9个连锁群,总长度502.9cM,标记间平均距离5.5cM。除α-胡萝卜素含量外,α-胡萝卜素、总胡萝卜素和番茄红素含量分别检测到1个主效QTL,均为加性遗传效应,分别解释表型变异为12.79%、12.87%和14.61%。此外,α-胡萝卜素和番茄红素含量还分别检测到1对上位性QTL,最大遗传效应分别为显性×加性互作和显性×显性互作,分别解释表型变异为15.1%和6.5%。文章中与QTL连锁的分子标记可用于高胡萝卜素、番茄红素的种质筛选和聚合育种。

英文摘要：

An F2 population derived from two carrot inbred lines,P50006 and HCM A.C. with high carotene accumulation,was developed and used to map and analyze quantitative trait locus（QTL） associated with the accumulation of α and β-carotene,total carotene and lycopene. Broad-sense heritabilities of these traits were 0.75,0.50,0.31,and 0.93,respec-tively. A genetic map with 91 SRAP（Sequence-related amplified polymorphism） markers was developed,which spanned 502.9 cM in 9 linkage groups with a mean marker interval of 5.5 cM. Mixed-model-based composite interval mapping was performed to analyze QTL and epistasis effects. One major QTL each for α-carotene,total carotene and lycopene accumula-tion were detected which can explain 12.79%,12.87%,and 14.61% of total phenotypic variations,respectively. Additive genetic variance was primarily responsible for genetic variability in all three major QTL. In addition,a pair of epistasis QTL for α-carotene and lycopene accumulation was detected,which were able to explain 15.1% and 6.5% of total phenotypicvariation,respectively. The dominant × additive and dominant × dominant interaction variance were primary epistasis effect for α-carotene and lycopene. These SRAP markers linked to QTL could be used in selection or QTL pyramiding for high carotene and lycopene content in carrot breeding.