中文摘要：

制备一种吖啶-4-酰胺类探针9-[（N-2-二甲氨乙基）吖啶-4-酰胺]-α-丙氨酸（NACA），研究NACA与牛血清蛋白（BSA）的结合作用．测定了Stern—Volmer猝灭常数K5v、热力学参数（△H、△G及△S）、结合位点数n、表观结合常数KA和NACA-BSA作用距离r，确定了NACA对BSA的荧光静态猝灭机制和主要作用力．

英文摘要：

An acridine - 4 - carboxamide skeleton - based probe N - （9 - （ N - （ 2 - dimethylamino） ethyl） acridine - 4 - carboxamide） - α - alanine （NACA） was synthesized. The interaction between NACA and bovine serum albumin （BSA） was investigated. The Stern - Volmer fluorescence quenching constant （Ksv） was investigated, as well as thermodynamics constants of △H, △G and △S. Other param- eters including the number of combining sites （ n）, apparent combining constants （ KA ） and interaction distance （r） of NACA -BSA were estimated. The static quenching was found to cause the fluorescence quenching of BSA and the interaction mechanism between NACA and BSA was also discussed.