中文摘要：

目的以具有产石杉碱甲能力的蛇足石杉离体叶状体为研究对象,探究蛇足石杉离体培养物累积石杉碱甲的影响因素及生产动态变化规律。方法应用正交设计对蛇足石杉离体培养叶状体的植物生长物质浓度进行筛选,采用HPLC法对叶状体提取物中的目标成分进行定性定量检测,用Logistic模型拟合叶状体的生长曲线。结果以白光为主要光源,光照时间为12 h/d,1/4 MS固体培养基,为蛇足石杉叶状体产石杉碱甲的最佳条件,相对增殖率达到3 174.5%,石杉碱甲累积量为72.45μg/L;蛇足石杉离体叶状体增殖的生长曲线呈＂S＂形,继代周期以50 d左右为最适;60 d后生长进入稳定阶段,累积石杉碱甲量明显加快,直至85 d达到最大值7.453μg/g,即叶状体先快速增殖,后累积石杉碱甲。结论叶状体累积石杉碱甲与叶状体增殖呈非生长偶联型,叶状体增长规律符合Logistic生长模型,最大的比生长速率（μmax）为0.071·d-1。

英文摘要：

Objective To investigate the influence factors and dynamic varitation rule for the accumulation of huperzine A（Hup A） from thallus of Huperzia serrata,which could produce Hup A.Methods The optimal concentration of plant growth substances for the growth of thallus of H.serrata were screened using orthogonal design method.The content of target extract from thallus of H.serrata were tested using HPLC.The kinetic curve of thallus growth was described using Logistic growth model.Results The thallus of H.serrata could produce the most Hup A（72.45 μg/L） when cultured in 1/4 MS solid medium complemented with 12 h/d white light and the relative growth rate could reach 3 174.5%.According to the growth curve of thallus of H.serrata like ＂S＂ shape,the best successive generation date of callus culture of H.serrata was 50 d.The concentration of Hup A increased obviously after 60 d when the thallus reached the stable period and the maximal value of Hup A was 7.453 μg/g at 85 d,which meant that the rapid growth of thallus was priority to the accumulation of Hup A.Conclusion The relational model between the accumulation of Hup A from thallus and its growth is non-growth conjugation.In addition,the growth law of thallus is consistent with Logistic growth model and the maximum specific growth rate（μmax） was 0.071 d-1.