中文摘要：

病程相关蛋白（Pathogenesis related proteins,PRPs）是植物在受到病原菌侵染或者非生物胁迫时大量分泌的一种参与协同防御的蛋白。将莜麦病程相关蛋白permatin基因克隆到原核表达载体pET32L,构建pET32Lpermatin重组子,转化大肠杆菌E.coli BL21（DE3）菌株表达,经Heparin Sepharose fast flow离子柱、Sephacryl S-200凝胶柱纯化获得分子量大小为82kDa的电泳纯的NusA-Permatin。通过滤纸片法及荧光显微镜分析重组蛋白对尖孢镰刀菌（Fusarium oxysporum）的影响,结果表明：Permatin能够显著抑制F.oxysporum菌丝生长,导致F.oxysporum菌丝体形态异常。此外,DCFH-DA荧光探针检测显示,Permatin蛋白作用尖孢镰刀菌的初期引起菌丝体活性氧含量的升高。这些结果表明Permatin蛋白不仅能抑制尖孢镰刀菌生长,还能引起菌体形态变化和细胞体内活性氧水平的升高。

英文摘要：

Pathogenesis-related proteins （PRPs） are the products accumulated by plants when infected by phytopathogenic pathogens and abiotic stress, permatin gene belonging to PRPs from Arena nuda was inserted into pET32L vector and the recombinant plasmid pET32L-permatin was transferred into E. coli strain BL21（DE3）, and expressed by IPTG induction. NusA-permatin of molecular weight 82 kDa was efficiently purified by Heparin Sepharose fast flow and Sephacryl S-200 gel filtration. The antifungal activity of NusA-Permatin against Fusarium oxysporum was evaluated by filter paper dispersion and morphologic observation. The results indicated that NusA-Permatin showed a remarkable antifungal activity and distort mycelial membrane. In addition, the reactive oxygen species （ROS） of Fusarium oxysporum was accumulated by treatment with NusA-permatin, which detected by fluorescent probe DCFH-DA. These results suggest that NusA-Permatins not only inhibit the growth of Fusarium oxysporum, but also damage its membrane or raise ROS in mycelia.