中文摘要：

应用Dynal磁珠一生物素标记的微卫星探针（AC）8，（AG）8和（ATG）12与文冠果基因组DNA酶切片段杂交，捕获含有微卫星序列的DNA片段，连接到pMD18-T载体上，转入感受态细胞Trans5ct，构建文冠果富集微卫星文库．利用M13F和M13R载体序列引物筛选文库，对插入片段长度为400～1500bp的克隆进行测序．共获得123条序列，55条（44．7％）舍有微卫星位点，其中完美型占63．7％，非完美型22．4％，混合型10．3％．微卫星重复基元中，二核苷酸（AC）n最为常见．

英文摘要：

The DNA fractions containing microsatellite sequences were captured by hybridizating the digested Xanthoceras sorbifolia genomic DNA fragments with the oligonucleotide probes （AC） s, （AG） s and （ATG）12 attached to striptavadin coated magnetic beads （Dynal）. The enriched DNA fragments were ligated into pMD18-T easy vector and then transformed into competent TransSα cell to form an en- riched microsatellite sequence library. PCR screening using MI3 Forward and M13 Reverse as primers identified 123 clones containing 400 to 1 500 bp inserts. Sequence analysis of these positive clones confirmed 55 microsateUite sequences, with an enrichment efficiency of 44.7%. Of these microsatel- lite loci, 63.7% were perfect repeat motif, 22.4% imperfect and 10.3 compound repeat motifs. A- mong them, （AC）, was the most common motif.