中文摘要：

为了调查毒素蛋白免疫下大蜡螟Galleria mellonellaL．产生的免疫相关蛋白，采用双向电泳的方法，从大蜡螟血淋巴中鉴定得到一种昆虫肽聚糖识别蛋白Gm21，通过RT．PCR、cDNA末端快速扩增（RACE）技术克隆得到其全长基因，该基因全长cDNA具有完整的编码框，编码211个氨基酸，序列分析表明该蛋白是一种具有潜在酰胺酶活性的S型肽聚糖识别蛋白。本研究中Gm21蛋白在免疫压力下的上调表达及其cDNA序列的克隆，对进一步深入研究昆虫肽聚糖识别蛋白的功能具有重要的意义。

英文摘要：

An investigation was made of the immune protein in Galleria melloneUa L. that had been immune-challenged with toxic protein. A peptidoglycan-recognition protein, Gm21, in hemolymph of G. mellonella was identified by two dimensional （2-D） electrophoresis, and the encoding gene was cloned by RT-PCR and RACE. The full length cDNA has the complete open reading frame and encodes a protein of 211 amino acids. Sequence analysis indicated that the protein is a peptidoglycan-recognition protein （PGRP） -S-like protein with potential amindase activity. The increased expression of Gm21 protein in immune-challenged G. mellonella and molecular cloning of the Gm21 gene are important techniques for further study of peptidoglycan-recognition proteins.