中文摘要：

目的 利用小动物呼吸机的微量机械通气技术建立基于新生大鼠的胎粪吸入综合征动物模型。方法 取25只5~7日龄健康新生大鼠,1只作为正常对照;采用配对比较法将24只大鼠分为胎粪吸入性肺损伤模型组（MAS组）和假手术组（NS组）,保证造模成功后每组10只,分别于机械通气条件下气管内灌入胎粪或生理盐水。分析各组动物的血气分析、肺部X线表现、肺的湿/干质量比以及肺组织病理学情况。结果 MAS组p H值、氧分压均显著低于NS组[（7.19±0.11）vs（7.31±0.08）,P〈0.05;（25±9）vs（62±12）mm Hg,P〈0.05]。MAS组肺部X线出现胎粪吸入改变发生率显著高于NS组（10/10 vs 1/10）。MAS组肺湿/干质量比也显著高于NS组[（5.85±0.47）vs（4.28±0.71）,P〈0.05]。MAS组病理学改变符合胎粪吸入综合征表现,NS组双肺肺泡通气均一,无明显充血水肿。结论 在微量通气条件下利用新生大鼠制备胎粪吸入综合征模型是可行的,可以为新生儿肺损伤的基础研究提供稳定的小动物模型。

英文摘要：

Objective To establish an animal model of newborn meconium aspiration syndrome （MAS） by tiny volume ventilation with small animal ventilator. Methods Twenty-five healthy newborn rats （5- 7-day-old） were randomly assigned to MAS group and sham group （normal saline, NS ）, with l0 animals in each group after model establishment, and the left 1 rat served as normal control. The rats from the MAS group were inflicted with meeonium infusion via endotraehea tube, and those of the sham group were given infusion of normal saline. All rats were analyzed with blood gas analysis, lung X-ray filming, wet/dry ratio and pathological examination. Results The pH value and partial pressure of oxygen were significantly lower in the MAS than the NS groups （7.19 ±0.11 vs 7.31 ±0.08, 25 ±9 vs 62 ±12 mmHg, P 〈0.05）. X-ray filming showed that the rate of meconium aspiration was higher in the MAS than the NS groups （ 10/10 vs 1/10）. And the wet/dry ratio was significantly higher in the MAS rats than the NS rats （5.85± 0.47 vs 4.28± 0. 71, P 〈 0.05 ）. The MAS rats displayed typical pathological changes of MAS, while the NS rats showed uniform lung alveolar ventilation, without obvious edema. Conclusion It is feasible to establish MAS model under tiny volume ventilation in newborn rats. And the model can provide a stable small animal model for neonatal lung injury.