中文摘要：

建立了绵羊（Ovis aries）粒细胞-巨噬细胞集落刺激因子基因（GM-CSF）的EvaGreen Real-time PCR方法,具有较高的灵敏性（检测精度在10个拷贝数以内）和特异性（溶解曲线仅出现一个产物峰）。绵羊GM-CSF在脂多糖（LPS）诱导前不表达,在诱导4h后逐渐增加,在8h达到最高（470930拷贝数/ng总RNA）,在诱导16h之后表达水平逐渐降低。表明Real-time PCR是一种灵敏、可靠的检测绵羊GM-CSF表达的方法,GM-CSF参与早期的免疫应答反应。

英文摘要：

EvaGreen （similar to SYBR-Green） Real-time PCR method for detecting ovine granulocyte-macrophage colony stimulating factor （GM-CSF） was established. Dynamic expression of GM-CSF was investigated in lipopolysaccharide（LPS）stimulus alveolar macrophages（AMs）. The results showed that GM-CSF did not express before LPS stimulation, increased gradually in the 4 h after the induction and peak at 8 h（470 930 copies/ng total RNA） and decreased gradually after 16 h, indicating that the Real-time PCR is a sensitive and reliable method for analyzing the gene expression profiles of ovine GM-CSF and GM-CSF involves in the early immune response.