中文摘要：

目的：探讨补肾活血汤对雷公藤甲素诱导的卵巢储备功能下降秀丽隐杆线虫生育力的作用及其机制,以及秀丽隐杆线虫在生育力保护类药物功能筛选和早期评价中的应用价值。方法：将年轻成虫期秀丽隐杆线虫随机分为空白组、雷公藤甲素模型组和补肾活血汤治疗组（62.5、125、250 mg/ml）。雷公藤甲素构建秀丽隐杆线虫卵巢储备功能下降模型,补肾活血汤处理24、48和72 h后,统计后代数目,未受精卵母细胞数目,子宫内受精卵数目,有丝分裂区细胞数目,凋亡细胞和卵母细胞数目;RT-PCR测定细胞凋亡通路相关基因的mRNA表达水平。结果：与空白组比较,雷公藤甲素0.1μg/ml模型组秀丽隐杆线虫后代数目、子宫内受精卵数目、有丝分裂区细胞数目、卵母细胞数目显著减少,未受精卵母细胞和凋亡细胞数目显著升高,ced-3基因表达水平显著升高和ced-9基因表达水平显著下降。补肾活血汤处理卵巢储备功能下降线虫72 h后,与雷公藤甲素模型组相比,补肾活血汤250 mg/ml组线虫后代数目、子宫内受精卵数目、有丝分裂区细胞数目、卵母细胞数目显著增多,未受精卵母细胞和凋亡细胞数目显著降低,ced-3基因表达水平显著降低和ced-9基因表达水平显著增高。结论：补肾活血汤对雷公藤甲素致秀丽隐杆线虫卵巢储备功能下降有显著治疗作用,通过促进不同发育阶段卵细胞的发育而提高生育力,生殖腺细胞凋亡关键基因表达水平的改变是其发挥作用的重要机制之一。秀丽隐杆线虫可用于生育力保护类药物的功能筛选和早期评价。

英文摘要：

Objective： To study effects of Bushen Huoxue Tang（ BSHXT,Decoction for Reinforcing Kidney and Activating Blood） on the fertility of C. elegans in Triptolide-induced diminished ovarian reserve（ DOR） model. Methods： Young adult hermaphrodites were randomly allocated into 5 groups,namely the control group,the model group（ Triptolide,0. 1 μg/ml）,and BSHXT groups（ 62. 5,125,250 mg/m L）.DOR model of C. elegans was established by Triptolide. After the DOR hermaphrodites were treated by BSHXT for 24,48 and 72 h,the brood size,the number of unfertilized oocytes,the number of zygotes in uterus,the number of mitotic germ cells,the number of apoptotic cells in pachytene,the number of oocytes in diakinesis,and the relative mRNA expression levels of genes in apoptosis pathway were observed. Results： Compared with the control group,hermaphrodites in the model group showed significant decreases in brood size,the number of zygotes in uterus,the number of mitotic germ cells and the number of oocytes in diakinesis,and significant increases in the number of unfertilized oocytes and apoptotic cells in pachytene,accompanying with the increase of relative mRNA expression level of ced-3 and decrease of ced-9（ P〈0. 05）. After treated with BSHXT for 72 h,hermaphrodites in 250 mg/m L group showed significantly increases in brood size,the number of zygote in uterus,the number of mitotic germ cells and the number of oocytes in diakinesis,and decreases in the number of unfertilized oocytes and apoptotic cells in pachytene,accompanying with the decrease of the relative mRNA expression level of ced-3 and increase of ced-9（ P〈0. 05）,compared with the model group. Conclusion： BSHXT has significantly beneficial effect on the DOR induced by Triptolide in C. elegans mainly through promoting the development of oocytes in different developmental stages,resulting in the increased fertility,and the apoptosis pathway may play a crucial role in regulating the effect of BSHXT.