中文摘要：

目的探讨抗肿瘤相关糖蛋白-72（TAG-72）抗原的表达及其与原发性胆囊癌（PGC）病理特征的关系。方法用原核表达获得抗TAG-72单链抗体，并用聚丙烯酰胺凝胶电泳（SDS—PAGE）和Western blot得以验证；用免疫组化方法检测TAG-72抗原在PGC的表达。结果通过原核表达获取大小为31kDa的抗TAG-72单链抗体并得以证实。TAG-72抗原在胆囊良、恶性组织阳性率分别为59．6％、6．7％（P〈0．05）。高分化PGC的TAG-72抗原表达显著低于中低分化PGC（P〈0．05）；肿瘤直径≤2cm者TAG-72表达显著低于〉2cm者（P〈0．05）；在PGC Nevin临床分期Ⅰ～Ⅲ期与Ⅳ～Ⅴ期间则无显著性差异（P〉0．05）。结论获得了抗TAC-72单链抗体的原核表达方法。TAG-72抗原可以作为一个肿瘤标志物在临床中应用，为抗TAG-72单链抗体在PGC诊治中的应用奠定实验基础。

英文摘要：

[Objective] To investigate generation of prokaryotic expressions of anti-tumor associated glycoprotein-72 （TAG-72）scFv, and to detect TAG-72 expression in human primary gallbladder carcinomas （PGC）. [Methods] Anti-TAG-72 scFv was prokaryotic-expressed and proved to be 31 kDa fuse-protein by SDS- PAGE and Western blot. Exporesstion of TAG-72 antigen was determined in gallbladder tumor by immuriohistochemistry. [Result] The positive rates of TAG-72 in 47 PGCs and 15 gallbladder adenomahyperplastics were 59.6%and6.7%, respectively. It shows that TAG-72 expression in PGC was significantly higher than those ingallbladder adenoma-hyperplastics （ P〈0. 05） ; and that expression in well differentiated PGC （48. 3 %） was significantly lower than those in moderately,poorly differentiated PGC（48. 3%, P 〈 0.05）. TAG-72 was more highly expressed in smaller PGC （26.3%）than larger one （82.1%,P 〈 0. 05）,but no statistical difference between various PGC clinical stages. [Conclusion] We generated anti-TAG-72 scFv by prokaryotic expression and detected TAG-72 expression in human gallbladder tumors, it suggests that TAG-72 could be used as gallbladder tumor＇s maker in clinical applications.