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中文摘要：
用铅离子的特异性核酸适体（Aptamer）修饰金-钌（AuRu）复合纳米微粒（Au与Ru的摩尔比为5：1）制备了铅离子的核酸适体纳米探针（AptAuRu）.在pH=7.0的Na2HPO4-NaH2PO4缓冲溶液及85mmol/LNaCl存在下,AptAuRu纳米探针不聚集.存在的Pb2＋可与探针中的Aptamer形成较稳定的G-四分体结构,从而释放出AuRu复合纳米微粒并进一步聚集形成较大的微粒,导致592nm处的共振散射光强度增大.该反应液经0.15μm滤膜过滤后,获得未反应的AptAuRu滤液.滤液中的纳米微粒对氯酸钠-碘化钠反应具有较强的催化作用,其产物与阳离子表面活性剂形成缔合微粒,在472nm处有一较强的共振散射峰.随着Pb2＋浓度的增大,滤液中金-钌纳米微粒浓度降低,其催化作用减弱,共振散射强度降低.Pb2＋浓度在0.12～60pmol/L范围内与其共振散射强度降低值（ΔI472nm）呈线性关系,回归方程及相关系数分别为ΔI472nm=3.1c＋7.3和0.9967,Pb2＋检出限为0.08pmol/L.将本法用于废水中Pb2＋的检测,结果令人满意.
英文摘要：
The Au/Ru nanoparticle,in a molar ratio of 5：1 of Au to Ru,was modified by aptamer to prepare an aptamer AuRu nanoprobe（AptAuRu） for Pb2＋,and the AptAuRu probe was stable in pH=7.0 Na2HPO4-NaH2PO4 buffer solution and in the presence of 85 mmol/L NaCl.Upon addition of Pb2＋,the nanoprobe reacted with Pb2＋ to form very stable G-quadruplex,and the released AuRu nanoparticles aggregated to big particles,which resulted in the resonance scattering（RS） peak at 592 nm increasing.The increased RS intensity was linear to the concentration of Pb2＋ in the range of 1.2-240 pmol/L.After filtration,the unreacted AptAuRu nanoprobe in filtration solution exhibited strong catalytic effect on the slow particle reaction of NaClO3-NaI-TDMAC,and the association complex particle appeared a resonance scattering peak at 472 nm.When the concentration of Pb2＋ increased,the amount of AptAuRu nanoprobe in filtration solution decreased,and the catalysis weak,that result to the resonance scattering intensity at 472 nm decreasing.The decreased intensity ΔI472 nm was linear to the Pb2＋ concentration in the range of 0.12-60 pmol/L,with a regression equation of ΔI472 nm=3.1c＋7.3,and a detection limit of 0.08 pmol/L Pb2＋.The RS assay was applied to the analysis of Pb2＋ in waste water,with a satisfactory result.
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