以病叶率、病情指数和鲜重抑制率为评价指标,研究生防真菌的接种体和杂草的发育阶段对草茎点霉菌SYAU-06侵染鸭跖草的影响。以浓度为1.03×109 cfu/m L菌丝体悬浮液接种,鸭跖草的发病率、病情指数、鲜重抑制率分别为91.16%、74.26和53.35%;相同浓度的分生孢子和厚垣孢子接种引起鸭跖草发病率为56.62%和41.58%,病情指数25.76和21.02,鲜重抑制率为15.39%和11.96%。菌丝体的接种浓度要高于107 cfu/m L,培养时间控制在7~9 d,寄主植物鸭跖草处于3叶期前,可以利用草茎点霉菌SYAU-06成功控制鸭跖草危害。
The weed dayflower Commelina communis may be suppressed in the infection of Phoma herbarum. We studied the effects of fungal inoculum and host leaf stage on the infection of fungal strain SYAU-06. Experiments showed that inoculation with mycelial suspension at 1.03×109 cfu/mL gave rise to infected leaf rate, disease index and fresh weight reduction at 91.16%, 74.26 and 53.35%, respectively. Inoculation with conidia and chlamydospore at the same inoculation concentration caused 56.62%and 41.58%infected leaf rate, 25.76 and 21.02 disease index, 15.39%and 11.96%fresh weight reduction. Perfect control of the weed with strain SYAU-06 was achieved by inoculation with mycelial suspension over 107 cfu/mL before 3-leave stage and 7-9 days of culture time.