应用石蜡切片方法和徒手切片方法,通过对水稻幼苗根系通气组织发育的观察,比较两种切片的异同.结果显示:石蜡切片和徒手切片都能清楚显示水稻根系通气组织的结构;并由此确定了水稻幼根通气组织形成过程,具体为:4天苗龄的水稻幼苗根系在培养24 h后开始形成通气组织,72 h时通气组织发育成熟.越靠近根基的部位,植株组织发育更成熟,通气组织的发育程度也更高.相比于石蜡切片,徒手切片方法更适用于水稻根系通气组织结构的观察.进一步利用徒手切片方法比较水稻和小麦根系通气组织形成对低氧的反应,发现低氧显著诱导麦根通气组织的形成,而对稻根通气组织发育的促进作用不明显.
We observed the aerenchyma formation in rice primary root and compared the images obtained by paraffin section and freehand section methods.The results showed that,both of the section types were able to display the structure of rice root and the formation of aerenchyma tissue clearly.The aerenchyma in roots of 4-d old rice seedlings began to form after 24 h growth,and came to completely mature by 72 h.The more closer to the root basal portion,the more mature of the tissue,consequently the higher degree of the aerenchema development.Compared to paraffin section method,the freehand section method is more suitable for observation of the architecture of aerenchyma in rice root.Aerenchyma production in rice and wheat root upon hypoxia observed by freehand section method showed that hypoxia can significantly induce aerenchyma formation in wheat roots,whereas the promoting function in rice roots is not significant.