中文摘要：

目的：探讨308 nm准分子激光对人角质形成细胞（hKCs）活力及碱性成纤维细胞生长因子（bF-GF）表达与分泌的影响。方法：体外培养人角质形成细胞,免疫荧光法鉴定细胞。分别予不同剂量308 nm准分子激光及311 nm窄谱中波紫外线（NB-UVB）照射,MTT法检测hKCs细胞活力,RT-PCR法和ELISA法分别检测bFGF表达和分泌水平,并与NB-UVB进行对比。结果：细胞鉴定证实为角质形成细胞。308 nm准分子激光剂量达600 mj/cm2,NB-UVB剂量达500 mj/cm2时hKCs活力下降;2种光源在一定剂量范围内照射均可使hKCs表达、分泌bFGF水平增加,并呈剂量依赖性;308 nm准分子激光诱导hKCs分泌bFGF的水平高于NB-UVB。结论：308 nm准分子激光照射对hKCs活力的影响小于NB-UVB;308 nm准分子激光可以通过剂量依赖的方式诱导hKCs表达与分泌bFGF来促使白癜风复色,与NB-UVB相比效果更佳。

英文摘要：

AIM： To investigate the effect of 308 nm excimer laser on the cell viability,and mRNA expression and protein secretion of basic fibroblast growth factor（bFGF） in human keratinocytes（hKCs）.METHODS： Human keratinocytes cultured in vitro were identified by immunofluorescence method and radiated with 308 nm excimer laser and 311 nm narrow-band ultraviolet B（NB-UVB） at different dosages,respectively.MTT method was used to measure the cell viability.The mRNA expression and protein secretion of bFGF were detected by the methods of RT-PCR and ELISA,respectively.The cells treated with NB-UVB were used for comparison.RESULTS： The cytoplasm of the cells presented kelly fluorescence under the fluorescent microscope,indicating that the cells were keratin positive.The cell viability were decreased by the exposure to 600 mj/cm2 of 308 nm excimer laser or 500 mj/cm2 of NB-UVB.The inhibitory effect of 308 nm excimer laser on the cell viability was slighter than that of NB-UVB.In both 308 nm excimer laser group and NB-UVB group,the mRNA expression and protein secretion of bFGF were significantly higher than those in control group and increased in a dose-dependent manner.The cells in 308 nm excimer laser groups had higher secretion level of bFGF than that in NB-UVB groups in certain range of dosages.CONCLUSION： The effect of 308 nm excimer laser on the cell viability is better than that of NB-UVB.The upregulation of bFGF production in keratinocytes by 308 nm excimer laser is in a dose-dependent manner,so as to promote the repigmentation of vitiligo lesions.