中文摘要：

为评价水体中低浓度壬基酚（NP）对中华大蟾蜍（Bufo bufogargarizans）蝌蚪的毒性效应,采用室内饲养、测定方法,研究了NP在不同暴露时间（8、16、24、32d）,不同浓度（0.002、0.005、0.010mg·L-1）条件下,对蝌蚪生长发育、过氧化氢酶（CAT）活性、丙二醛（MDA）含量和DNA损伤的影响。结果表明：暴露于0.010mg·L-1NP的蝌蚪,其生长发育被极显著抑制（P〈0.01）;CAT活性在两个较低浓度组（0.002、0.005mg·L-1）表现为先诱导后恢复,在0.010mg·L-1浓度组表现为诱导-恢复-诱导（P〈0.05）;MDA含量在两个较低浓度组（0.002、0.005mg·L-1）持续升高（P〈0.05）,呈明显的时间-效应关系;各处理组DNA损伤水平均显著高于对照组（P〈0.05）,但随暴露时间的延长而有所下降,具有一定的剂量-效应关系。总之,即使水体中的NP符合灌溉标准,也可能抑制蝌蚪的生长发育,并对其造成氧化损伤和遗传损伤。

英文摘要：

There were few reports on the toxic effects of nonylpheno（lNP）on tadpoles.This research was designed to investigate the development,catalase（CAT）activity,content of malonyldialdehyde（MDA）and DNA damage of Bufo bufogargarizans tadpoles exposed to low concentration of NP.The tadpoles were exposed to NP concentrations of 0.002,0.005 and 0.010 mg·L-1 for a period of 8,16,24 and 32 d.The results were as follows：（1）The development of tadpoles was inhibited significantly during exposure to 0.010 mg·L-1 NP（P0.01）.（2）The CAT activity was induced initially and then depressed to control level at the concentrations of 0.002 and 0.005 mg·L-1,while the CAT activity was first induced,and then depressed,but induced again at the concentration of 0.010 mg·L-（1P0.05）.（3）The content of MDA increased gradually from 24 to 32 d after exposure to 0.002 and 0.005 mg·L-1 NP（P0.05）,and there existed a time-effect relationship.（4）Compared with the control group,the level of DNA damage increased to the top value at 16 d（P0.05）,displaying a dose-effect relationship,but it decreased as the exposure time prolonging.In conclusion,even at the concentration lower than the irrigation standard,NP still inhibited the development of tadpoles,and resulted in oxidative injury and DNA damage.