中文摘要：

肿瘤标志物对于肺腺癌病人的临床诊断和预后具有重要意义.本研究根据临床诊断选取人肺腺癌组织和癌旁正常肺组织为研究对象,采用差速离心联合双水相法纯化组织细胞质膜,运用同位素标记相对和绝对定量技术结合高效液相色谱-串联质谱技术,鉴定出肺腺癌组织和癌旁正常肺组织的差异蛋白质41种.同癌旁正常肺组织相比,18个蛋白质在肺腺癌组织中表达上调,23个蛋白质在肺腺癌组织中表达下调.生物信息学分析发现,差异质膜蛋白质FLOT1、CAV1和ITGB1均处于蛋白质相互作用网络的重要位置,可能参与了肺腺癌相关信号转导.利用蛋白质印迹和免疫组织化学染色验证,差异蛋白质FLOT1、CAV1和ITGB1在肺腺癌织和癌旁正常肺组织的表达情况,其验证结果与蛋白质组学研究结果一致.研究结果对肺腺癌诊断标志物和肺腺癌癌变分子机理研究具有重要意义.

英文摘要：

Tumor markers are of great significance in the clinical diagnosis and prognosis for lung adenocarcinoma patients. In this study,lung adenocarcinoma tissues and paired paraneoplastic normal lung tissues（ PNLTs） were collected for investigation. Differential centrifugation and aqueous two-phase partition technology was used to purify the plasma membrane（ PM）. HPLC-MS / MS was used in combination with i TRAQ（ isobaric tag for relative and absolute quantitation） for analysis. Differentially expressed PM proteins of 41 in total between primary lung adenocarcinomas and PNLTs were identified,including 18 up-regulated and 23 down-regulated in cancer tissues. Bioinformatic analysis showed that the identified FLOT1,CAV1 and ITGB1 were involved in protein-protein interaction network in the signal transduction of lung adenocarcinoma. The expressions of FLOT1,CAV1 and ITGB1 were detected by Western blotting and immunohistochemistry for validation.