中文摘要：

以玉米（ZeamaysL．）为材料，探讨了水分胁迫下玉米钙调素（CaM）基因CaM1、CaM2和CaM3在叶片中转录水平的变化；并对水分胁迫下脱落酸（ABA）积累和H2O2产生与CaM基因表达的关系进行了研究。结果表明，25％PEG6000模拟的水分胁迫能够诱导上述玉米CaM基因在叶中的表达，其中对CaM1和CaM3的影响较为显著，3个基因具有各自不同的表达模式。外源ABA处理也能显著诱导这3个基因的表达，施用ABA合成抑制剂FLU明显抑制了水分胁迫下CaM基因表达，暗示水分胁迫下CaM基因表达增强依赖于ABA的积累。外源H2O2处理下3个CaM基因均出现2次表达增强的情况，ROS抑制剂或清除剂并不影响外源ABA诱导的CaM基因的前期表达，仅抑制了后期表达，暗示H2O2参与ABA诱导的CaM基因表达后期的调控，它们之间可能存在交谈机制，并且这种机制在不同CaM基因之间具有普遍性。

英文摘要：

The expression pattern of three known maize calmodulin （CaM） genes CAM1, CaM2 and CaM3 was investigated in leaves of maize （ Zea mays L. ） plants exposed to water stress induced by polyethylene glycol （ PEG 6000 ） and the relationship between the expression of CaM genes and the accumulation of abseisic acid （ABA） and hydrogen peroxide （H2O2 ） induced by water stress was studied. Treatments with PEG （25%） and ABA led to the expression of three CaM genes in leaves of maize. Pre- treatment with the ABA biosynthetic inhibitor fluridon （FLU） substantially blocked the expression of the CaM genes induced by wa- ter stress, suggesting that the increased expression of CaM genes under water stress is associated with the accumulation of ABA. There appeared two peaks in the expression of CaM genes in leaves of maize plants exposed to H2O2 treatment. Pretreatments with H2O2 scavenger or NADPH oxidase inhibitor substantially reduced the ABA-induced increases in the expression of the CaM genes after the maximum values, but only had a very little effect on the expression before the maximum values. These results suggest that ABA and H2O2 are required for water stress-induced expression of the CaM genes in leaves of maize plants, and there is a cross-talk between ABA, CaM and H2O2 under water stress.