中文摘要：

用不同质量浓度GA3浸泡天女木兰种子并结合变温层积处理,应用高效液相色谱法对不同时期种子中4种激素GA3、IAA、ABA、ZR含量进行测定,并测量种胚长和萌发率,以探讨天女木兰种胚发育,内源激素含量变化与种子休眠萌发之间的调控关系,为进一步研究种子休眠机理提供理论基础.结果表明：（1）天女木兰成熟种子胚发育不完全,胚乳内高浓度ABA和低浓度GA3是其休眠的主要原因.（2）GA3处理能促使天女木兰种子提前30d完成形态后熟,并以1 500 mg·L^-1 GA3处理效果最佳.（3）在变温层积过程,天女木兰种胚发育可分三个阶段：阶段Ⅰ （0~70 d）完成种胚进一步分化;阶段Ⅱ（70~120 d）种胚快速生长时期;阶段Ⅲ（120~150 d）休眠完全解除,种子具备发芽能力.种子能否打破休眠主要取决于阶段Ⅰ和Ⅱ的状况.（4）GA3/ABA、IAA/ABA和ZR/ABA在种子后熟期间的变化同胚生长发育存在一致性,认为内源激素的相对水平对种子休眠具有重要的调控作用.

英文摘要：

Through soaking seeds with different concentrations of GA3 combination of variable temperature stratifications,we analyzed the content of four hormones（GA3,IAA,ABA,ZR） in seeds of different periods by high performance liquid chromatography and measuring the embryo length and seed germination rate of M.sieboldii,to learn the relationship among the development of embryo,the levels of endogenous hormones and the regulation of seed dormancy and germination,to provide a theoretical basis for further study of seed dormancy mechanism,to provide a theoretical basis for further research on seed dormancy mechanism.we came to certain conclusions as below：（1）Embryo of mature seeds of M.sieboldii developed incompletely and seed dormancy was mainly due to high concentration of ABA and low concentrations of GA3 ; （2）GA3 treatments could stimulate the morphological maturation of seeds by 30 days,and treatment with GA3 of 1 500 mg · L 1 saw the best effect; （3）The whole temperature stratification process could be divided into three stages：Stage Ⅰ （0-70 d）,this stage was to complete the further differentiate of the seed embryo;Stage Ⅱ （70-120 d） was the fast grow period of embryo;Stage Ⅲ （120-150 d）,seed dormancy was broken absolutely and seeds had the ability to sprout.Whether seeds can break dormancy depends on stage Ⅰ and stage Ⅱ ;（4）The changes of GA3/ABA,IAA/ABA and ZR/ABA consist of embryo growth and development during the ripening of the seeds.The relative level of hormones played an important role in seed dormancy regulation.