中文摘要：

首先将巴马小型猪（BM）、巴马香猪（BMXZ）外周血淋巴细胞分别与G418抗性HEK293细胞（G418^R293）共培养，通过G418加压筛选，除去共培养体系中巴马小型猪、巴马香猪外周血淋巴细胞，然后应用PCR及RT-PCR的方法对所制备的感染细胞模型进行系统鉴定。结果经6周共培养及3周加压筛选后，细胞的形态、生长速度及折光性均未见明显变化；PCR及RT-PCR鉴定表明，筛选后的共培养体系中已没有巴马小型猪、巴马香猪外周血淋巴细胞的存在；PERV特异性检测方法检测显示，该细胞模型的DNA中已有PERV的整合且有PERV特异性mRNA的表达。从而证实了猪外周血淋巴细胞来源的PERV在体外也能够感染人源细胞系，为研究PERV的生物学特性及病原安全性的评价搭建了技术平台。

英文摘要：

Based on HEK293 cell strains with G418 resistance, we co-cultured the cells and PBL of BM minipigs and screened by G418. Systematic evaluation was executed on the cell models by PCR and RT-PCR after removing of PBL. We constructed HEK293 cell models infected by BM PERV, and verified the infectivity of PERV from porcine PBL to human-derived cells in vitro, which helped in PERV biological characteristics and pathogen safety research.